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Western blot analysis of lysates from Hep G2 cells, using ST6GAL1/CD75 Rabbit mAb (A24645) at 1:7000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded Human liver using ST6GAL1/CD75 Rabbit mAb (A24645) at dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Confocal imaging of Daudi cells using ST6GAL1/CD75 Rabbit mAb (A24645,dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007,dilution 1:500)(Red).DAPI was used for nuclear staining (Blue). Objective: 100x.
Flow cytometry: 1X10^6 HeLa cells (negative control,left) and Daudi cells (right) were surface-stained with ST6GAL1/CD75 Rabbit mAb (A24645,2 μg/mL,orange line) or ABflo® 647 Rabbit IgG isotype control (A22070,2 μg/mL,blue line), followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).
Flow cytometry: 1X10^6 Daudi cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070,2 μg/mL,left) or ST6GAL1/CD75 Rabbit mAb (A24645,2 μg/mL,right).
Western blot analysis of lysates from Hep G2 cells, using ST6GAL1/CD75 Rabbit mAb (A24645) at 1:7000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Exposure time: 90s.

ST6GAL1/CD75 Rabbit Recombinant Antibody (82-968)

CATALOG NUMBER: 82-968

Clonality:
Monoclonal
Tested Applications:
ELISA, FC, IF/ICC, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, FC, IF/ICC, IHC-P, WB
Application Note:
WB,1:2000 - 1:8000, IHC-P,1:500 - 1:1000, IF/ICC,1:50 - 1:200, FC,1:500 - 1:1000, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Predicted Molecular Weight:
20kDa/47kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
ST6GAL1
Additional Names:
ST6N; CDw75; SIAT1; ST6GalI; ST6GAL1/CD75
Protein Accession Number:
P15907
Ncbi Gene Id Number:
6480
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of glycosyltransferase family 29. The encoded protein is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The protein, which is normally found in the Golgi but can be proteolytically processed to a soluble form, is involved in the generation of the cell-surface carbohydrate determinants and differentiation antigens HB-6, CD75, and CD76. This gene has been incorrectly referred to as CD75. Alternative splicing results in multiple transcript variants.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

82-968

List Size:
100 uL

List Price:

$477.00

Datasheet
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