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Western blot analysis of lysates from wild type (WT) and 293T transfected with Snail, using Snail Rabbit mAb at 1:9000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 5s.
Immunofluorescence analysis of 293F cells transfected with Snail,using Snail Rabbit mAb at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 300ug extracts from 293F cells transfected with Snail using 3ug Snail Rabbit mAb. Western blot was performed from the immunoprecipitate using Snail Rabbit mAb at a dilition of 1:9000.
Western blot analysis of lysates from wild type (WT) and 293T transfected with Snail, using Snail Rabbit mAb at 1:9000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 5s.

Snail Rabbit Recombinant Antibody (82-997)

CATALOG NUMBER: 82-997

Clonality:
Monoclonal
Tested Applications:
ELISA, IF/ICC, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF/ICC, IP, WB
Application Note:
WB,1:9000 - 1:54000, IF/ICC,1:200 - 1:400, IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Control 1:
293T transfected with Snail (Human)
Predicted Molecular Weight:
29kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
SNAI1
Additional Names:
SNA; SNAH; SNAIL; SLUGH2; SNAIL1; dJ710H13.1; Snail
Protein Accession Number:
O95863
Ncbi Gene Id Number:
6615
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The Drosophila embryonic protein snail is a zinc finger transcriptional repressor which downregulates the expression of ectodermal genes within the mesoderm. The nuclear protein encoded by this gene is structurally similar to the Drosophila snail protein, and is also thought to be critical for mesoderm formation in the developing embryo. At least two variants of a similar processed pseudogene have been found on chromosome 2.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

82-997

List Size:
100 uL

List Price:

$540.00

Datasheet
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