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Western blot analysis of various lysates using Syntaxin 6 Rabbit mAb (A26336) at 1:2000 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Exposure time: 60 s.
Western blot analysis of various lysates using Syntaxin 6 Rabbit mAb (A26336) at 1:2000 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Exposure time: 60 s.

Syntaxin 6 Rabbit Recombinant Antibody (83-290)

CATALOG NUMBER: 83-290

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
WB,1:1000 - 1:4000, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Control 1:
SK-BR-3, 293T, Mouse brain, Neuro-2a, Rat brain, PC-12
Predicted Molecular Weight:
29 kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
STX6
Protein Accession Number:
O43752
Ncbi Gene Id Number:
10228
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Enables syntaxin binding activity. Involved in several processes, including Golgi ribbon formation; retrograde transport, endosome to Golgi; and vesicle fusion. Acts upstream of or within endocytic recycling. Located in several cellular components, including early endosome; perinuclear region of cytoplasm; and trans-Golgi network. Part of SNARE complex.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

83-290

List Size:
100 uL

List Price:

$477.00

Datasheet
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