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Western blot analysis of lysates from wild type (WT) and 293T cells transfected with CD62E using CD62E Rabbit mAb at 1:1000 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 20 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit | .Exposure time: 30s.
Confocal imaging of 293T cells transfected with CD62E using CD62E Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.
Flow cytometry: 1X10^6 CHO cells (negative control,left) and CHO (Transfection,right) cells were surface-stained with CD62E Rabbit mAb or Rabbit IgG isotype control, followed by PE Goat anti-Rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).
Flow cytometry: 1X10^6 CHO (Transfection) cells were surface-stained with Rabbit IgG isotype control or CD62E Rabbit mAb, followed by PE Goat anti-Rabbit pAb staining.
Flow cytometry: 1X10^6 bEnd.3 cells (right) were surface-stained with CD62E Rabbit mAb or Rabbit IgG isotype control, followed by PE Goat anti-Rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).
Western blot analysis of lysates from wild type (WT) and 293T cells transfected with CD62E using CD62E Rabbit mAb at 1:1000 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 20 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit | .Exposure time: 30s.

CD62E Rabbit Recombinant Antibody (83-293)

CATALOG NUMBER: 83-293

Clonality:
Monoclonal
Tested Applications:
ELISA, FC, IF/ICC, WB
Host Species:
Rabbit
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Tested Applications:
ELISA, FC, IF/ICC, WB
Application Note:
WB,1:500 - 1:1000, IF/ICC,1:50 - 1:200, FC,1:500-1:1000, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Predicted Molecular Weight:
66kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
Buffer: PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
E-selectin
Additional Names:
Elam; CD62E; ELAM-1; LECAM2; E-selectin
Protein Accession Number:
Q00690
Ncbi Gene Id Number:
20339
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Predicted to enable several functions, including oligosaccharide binding activity; phospholipase binding activity; and sialic acid binding activity. Involved in positive regulation of leukocyte tethering or rolling. Acts upstream of or within positive regulation of leukocyte migration. Predicted to be located in several cellular components, including caveola; clathrin-coated pit; and perinuclear region of cytoplasm. Predicted to be integral component of plasma membrane. Predicted to be active in external side of plasma membrane and extracellular space. Is expressed in several structures, including adrenal gland; genitourinary system; incisor; integumental system; and limb segment. Human ortholog(s) of this gene implicated in IgA glomerulonephritis; brain ischemia; cerebrovascular disease; and coronary artery disease. Orthologous to human SELE (selectin E).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

83-293

List Size:
100 uL

List Price:

$477.00

Datasheet
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