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Western blot analysis of various lysates using CD31/PECAM1 Mouse mAb at 1:1000 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Negative control (NC): SK-BR-3 | Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using CD31/PECAM1 Mouse mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using CD31/PECAM1 Mouse mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using CD31/PECAM1 Mouse mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
Confocal imaging of paraffin-embedded Human placenta tissue using CD31/PECAM1 Mouse mAb followed by a further incubation with Cy3 Goat Anti-Mouse IgG (H+L) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
Western blot analysis of various lysates using CD31/PECAM1 Mouse mAb at 1:1000 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Negative control (NC): SK-BR-3 | Exposure time: 90s.

CD31/PECAM1 Mouse Recombinant Antibody (83-466)

CATALOG NUMBER: 83-466

Clonality:
Monoclonal
Tested Applications:
ELISA, IF-P, IHC-P, WB
Host Species:
Mouse
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF-P, IHC-P, WB
Application Note:
WB,1:1000 - 1:2000, IF-P,1:200 - 1:800, IHC-P,1:200 - 1:800, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Predicted Molecular Weight:
83kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
Buffer: PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
PECAM1
Additional Names:
CD31; PECA1; GPIIA'; PECAM-1; endoCAM; CD31/EndoCAM
Protein Accession Number:
P16284
Ncbi Gene Id Number:
5175
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

83-466

List Size:
100 uL

List Price:

$540.00

Datasheet
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