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Western blot analysis of various lysates using RIP3 Rabbit mAb at 1:7000 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Negative control (NC): Neuro-2a | Exposure time: 1s.
Western blot analysis of lysates from J774A.1 cells using RIP3 Rabbit mAb at 1:7000 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 45s.
Immunofluorescence analysis of 293T cells transfected with RIP3 using RIP3 Rabbit mAb at a dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of L-929 cells using RIP3 Rabbit mAb at a dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Western blot analysis of various lysates using RIP3 Rabbit mAb at 1:7000 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Negative control (NC): Neuro-2a | Exposure time: 1s.

RIP3 Rabbit Recombinant Antibody (83-559)

CATALOG NUMBER: 83-559

Clonality:
Monoclonal
Tested Applications:
ELISA, IF/ICC, WB
Host Species:
Rabbit
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF/ICC, WB
Application Note:
WB,1:6000 - 1:28000, IF/ICC,1:100 - 1:200, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Predicted Molecular Weight:
63kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
Ripk3
Additional Names:
Rip3; 2610528K09Rik
Protein Accession Number:
Q9QZL0
Ncbi Gene Id Number:
56532
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Enables NF-kappaB-inducing kinase activity and protein serine/threonine kinase activity. Involved in several processes, including execution phase of necroptosis; hematopoietic or lymphoid organ development; and regulation of programmed cell death. Acts upstream of or within several processes, including I-kappaB kinase/NF-kappaB signaling; positive regulation of intrinsic apoptotic signaling pathway; and positive regulation of protein deacetylation. Located in cytoplasm and nucleus. Is expressed in several structures, including alimentary system; hemolymphoid system gland; male reproductive gland or organ; primitive streak; and turbinate bone primordium. Orthologous to human RIPK3 (receptor interacting serine/threonine kinase 3).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

83-559

List Size:
100 uL

List Price:

$477.00

Datasheet
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