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Western blot analysis of lysates from wild type (WT) and Rbm7 knockout (KO) mouse testis using [KO Validated] Rbm7 Rabbit mAb (A27655) at 1:5000 dilution incubated at room temperature for 1.5 hours. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Exposure time: 60s. | <b>WB samples for antibody validation are kindly provided by Dr. Shaorong Gao.</b>
Immunohistochemistry analysis of paraffin-embedded Mouse lung tissue using [KO Validated] Rbm7 Rabbit mAb (A27655) at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using [KO Validated] Rbm7 Rabbit mAb (A27655) at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using [KO Validated] Rbm7 Rabbit mAb (A27655) at a dilution of 1:5000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Confocal imaging of paraffin-embedded Rat testis tissue using [KO Validated] Rbm7 Rabbit mAb (A27655, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
Confocal imaging of paraffin-embedded Rat colon tissue using [KO Validated] Rbm7 Rabbit mAb (A27655, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
Confocal imaging of paraffin-embedded Mouse testis tissue using [KO Validated] Rbm7 Rabbit mAb (A27655, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
Confocal imaging of paraffin-embedded Mouse colon tissue using [KO Validated] Rbm7 Rabbit mAb (A27655, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
Immunoprecipitation of Rbm7 from 300 µg extracts of Mouse testis tissue was performed using 0.5 µg of [KO Validated] Rbm7 Rabbit mAb (A27655). Rabbit IgG isotype control (AC005) was used to precipitate the Control IgG sample. IP samples were eluted with 1X reducing Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using [KO Validated] Rbm7 Rabbit mAb (A27655) at a dilution of 1:4000.
Western blot analysis of lysates from wild type (WT) and Rbm7 knockout (KO) mouse testis using [KO Validated] Rbm7 Rabbit mAb (A27655) at 1:5000 dilution incubated at room temperature for 1.5 hours. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Exposure time: 60s. | WB samples for antibody validation are kindly provided by Dr. Shaorong Gao.

[KO Validated] Rbm7 Rabbit Recombinant Antibody

CATALOG NUMBER: 83-542

Clonality:
Monoclonal
Tested Applications:
ELISA, IF-P, IHC-P, IP, WB
Host Species:
Rabbit
Species Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse, Rat
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF-P, IHC-P, IP, WB
Application Note:
WB,1:2500 - 1:10000, IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells, IF-P,1:200 - 1:1000, IHC-P,1:2000 - 1:8000, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Predicted Molecular Weight:
30kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
Rbm7
Additional Names:
1200007M24Rik; 1500011D06Rik
Protein Accession Number:
Q9CQT2
Ncbi Gene Id Number:
67010
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Predicted to enable 14-3-3 protein binding activity and RNA binding activity. Predicted to be involved in regulation of alternative mRNA splicing, via spliceosome and snRNA catabolic process. Located in nucleus. Orthologous to human RBM7 (RNA binding motif protein 7).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

83-542

List Size:
100 uL

List Price:

$477.00

Datasheet
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