Western blot analysis of various lysates using Acetyl-Histone H3-K23 Rabbit mAb at 1:1000 dilution incubated overnight at 4℃. HeLa, NIH/3T3 and C6 cells were treated with TSA (1 μM) at 37℃ for 18 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit.|Exposure time: 180s.

Immunofluorescence analysis of U2OS cells using Acetyl-Histone H3-K23 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Western blot analysis of various lysates using Acetyl-Histone H3-K23 Rabbit mAb at 1:1000 dilution incubated overnight at 4℃. HeLa, NIH/3T3 and C6 cells were treated with TSA (1 μM) at 37℃ for 18 hours. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Enhanced Kit. | Exposure time: 180s.

Acetyl-Histone H3-K23 Rabbit Recombinant Antibody (83-551)

CATALOG NUMBER: 83-551

Clonality:

Monoclonal

Tested Applications:

ELISA, IF/ICC, WB

Host Species:

Rabbit

Species Reactivity:

Human, Mouse, Other (Wide Range Predicted), Rat

Conjugate:

Unconjugated

Specifications

Host Species:

Rabbit

Species Reactivity:

Human, Mouse, Other (Wide Range Predicted), Rat

Immunogen:

Synthetic peptide. This information is considered to be commercially sensitive.

Conjugate:

Unconjugated

Tested Applications:

ELISA, IF/ICC, WB

Application Note:

WB,1:500 - 1:1000, IF/ICC,1:50 - 1:200, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Positive Control 1:

HeLa treated with TSA, NIH/3T3 treated with TSA, C6 treated with TSA

Predicted Molecular Weight:

16kDa

Advanced Validation

Properties

purification:

Affinity purification

Clonality:

Monoclonal

Isotype:

IgG

physical-state:

Liquid

Buffer:

Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.

Concentration:

Batch dependent

Storage Conditions:

Store at -20℃. Avoid freeze / thaw cycles.

Additional Info

Ncbi Official Symbol:

Histone H3

Additional Names:

H3/A; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FA; H3C10; H3C11; H3C12; HIST1H3A; H3C1; Acetyl-Histone H3-K23

Protein Accession Number:

Q16695/P68431

Ncbi Gene Id Number:

8290/8350

User Note:

Optimal dilutions for each application to be determined by the researcher.

Background

Background:

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Citations

Set Contents

Disclaimer

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:

This product is for research use only.

View product citations for catalog number 83-551 on CiteAb

CATALOG NUMBER:

83-551

List Size:

100 uL

List Price:

$540.00

Datasheet

Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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Acetyl-Histone H3-K23 Rabbit Recombinant Antibody (83-551)

Clonality:

Tested Applications:

Host Species:

Species Reactivity:

Conjugate:

Host Species:

Species Reactivity:

Immunogen:

Conjugate:

Tested Applications:

Application Note:

Positive Control 1:

Predicted Molecular Weight:

purification:

Clonality:

Isotype:

physical-state:

Buffer:

Concentration:

Storage Conditions:

Ncbi Official Symbol:

Additional Names:

Protein Accession Number:

Ncbi Gene Id Number:

User Note:

Background:

Disclaimer:

List Size:

Shipping Info

Customize your order

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