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Western blot analysis of various lysates using CRMP2/DPYSL2 Rabbit mAb (A4411) at 1:1000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Exposure time: 3s.
Western blot analysis of lysates from wild type(WT) and CRMP2/DPYSL2 knockout (KO) HeLa cells, using [KO Validated] CRMP2/DPYSL2 Rabbit mAb (A4411) at 1:1000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Exposure time: 1s.
Immunofluorescence analysis of Rat brain tissue using [KO Validated] CRMP2/DPYSL2 Rabbit pAb (A4411) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. Microwave antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining.
Immunofluorescence analysis of Mouse brain tissue using [KO Validated] CRMP2/DPYSL2 Rabbit pAb (A4411) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. Microwave antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining.
Western blot analysis of various lysates using CRMP2/DPYSL2 Rabbit mAb (A4411) at 1:1000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Exposure time: 3s.

[KO Validated] CRMP2/DPYSL2 Rabbit Recombinant Antibody

CATALOG NUMBER: 83-914

Clonality:
Monoclonal
Tested Applications:
ELISA, IF-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF-P, WB
Application Note:
WB,1:1000 - 1:5000, IF-P,1:50 - 1:200, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Control 1:
HeLa,Mouse lung,Mouse heart,Rat lung,Rat heart
Predicted Molecular Weight:
62kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
DPYSL2
Additional Names:
DRP2; N2A3; CRMP2; DRP-2; ULIP2; CRMP-2; DHPRP2; ULIP-2; L2
Protein Accession Number:
Q16555
Ncbi Gene Id Number:
1808
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the collapsin response mediator protein family. Collapsin response mediator proteins form homo- and hetero-tetramers and facilitate neuron guidance, growth and polarity. The encoded protein promotes microtubule assembly and is required for Sema3A-mediated growth cone collapse, and also plays a role in synaptic signaling through interactions with calcium channels. This gene has been implicated in multiple neurological disorders, and hyperphosphorylation of the encoded protein may play a key role in the development of Alzheimer's disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

83-914

List Size:
100 uL

List Price:

$540.00

Datasheet
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