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Western blot analysis of various lysates using Histone H2A.Z Rabbit mAb (A4599) at 1:1000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Exposure time: 30s.
Western blot analysis of lysates from wild type(WT) and Histone H2A.Z knockout (KO) 293T(KO) cells, using [KO Validated] Histone H2A.Z Rabbit mAb (A4599) at 1:1000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Exposure time: 1s.
Immunoprecipitation of Histone H2A.Z from 100 µg extracts of HeLa cells was performed using 3 µg of [KO Validated] Histone H2A.Z Rabbit mAb (A4599). Rabbit Control IgG (AC005) was used to precipitate the Control IgG sample. IP samples were eluted with 1x reducing Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using [KO Validated] Histone H2A.Z Rabbit mAb (A4599) at a dilution of 1:1000.
Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Histone H2A.Z Rabbit mAb antibody (A4599) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Western blot analysis of various lysates using Histone H2A.Z Rabbit mAb (A4599) at 1:1000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit (RM00020). | Exposure time: 30s.

[KO Validated] Histone H2A.Z Rabbit Recombinant Antibody

CATALOG NUMBER: 83-959

Clonality:
Monoclonal
Tested Applications:
ChIP, ELISA, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Other (Wide Range Predicted), Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Other (Wide Range Predicted), Rat
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ChIP, ELISA, IP, WB
Application Note:
WB,1:1000 - 1:5000, IP,0.5μg-4μg antibody for 100μg-300μg extracts of whole cells, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements., ChIP,5μg antibody for 5μg-10μg of Chromatin
Positive Control 1:
HeLa,293T,U-87MG,Mouse lung,Mouse testis,Mouse brain,Rat lung,Rat testis,
Predicted Molecular Weight:
14kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
H2AZ1
Additional Names:
H2AZ; H2A.z; H2A/z; H2AFZ; H2A.Z-1; .Z
Protein Accession Number:
P0C0S5
Ncbi Gene Id Number:
3015
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent member of the histone H2A family that is distinct from other members of the family. Studies in mice have shown that this particular histone is required for embryonic development and indicate that lack of functional histone H2A leads to embryonic lethality.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

83-959

List Size:
100 uL

List Price:

$540.00

Datasheet
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