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Western blot analysis of lysates from wild type (WT) and GATA2 knockout (KO) HeLa cells, using [KO Validated] GATA2/3 Rabbit mAb (A5083) at 1:1000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Enhanced Kit (RM00021). | Exposure time: 3min.
Western blot analysis of various lysates using [KO Validated] GATA2/3 Rabbit mAb (A5083) at 1:1000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Enhanced Kit (RM00021). | Exposure time: 3min.
Western blot analysis of lysates from wild type (WT) and GATA2 knockout (KO) HeLa cells, using [KO Validated] GATA2/3 Rabbit mAb (A5083) at 1:1000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Enhanced Kit (RM00021). | Exposure time: 3min.

[KO Validated] GATA2/3 Rabbit Recombinant Antibody

CATALOG NUMBER: 84-086

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
WB,1:500 - 1:1000, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Control 1:
HeLa,Mouse lung,Rat lung
Predicted Molecular Weight:
51kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
GATA2
Additional Names:
DCML; IMD21; MONOMAC; NFE1B; /3
Protein Accession Number:
P23769/P23771
Ncbi Gene Id Number:
2624/2625
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the GATA family of zinc-finger transcription factors that are named for the consensus nucleotide sequence they bind in the promoter regions of target genes. The encoded protein plays an essential role in regulating transcription of genes involved in the development and proliferation of hematopoietic and endocrine cell lineages. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Mar 2009]

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

84-086

List Size:
100 uL

List Price:

$540.00

Datasheet
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