Western blot analysis of various lysates using RUVBL1/TIP49A/PONTIN Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 3min.

Immunohistochemistry analysis of paraffin-embedded Mouse testis using RUVBL1/TIP49A/PONTIN Rabbit mAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

Chromatin immunoprecipitation analysis of extracts of HepG2 cells, using RUVBL1/TIP49A/PONTIN Rabbit mAb antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Western blot analysis of various lysates using RUVBL1/TIP49A/PONTIN Rabbit mAb at 1:1000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 3min.

RUVBL1/TIP49A/PONTIN Rabbit Recombinant Antibody (84-122)

CATALOG NUMBER: 84-122

Clonality:

Monoclonal

Tested Applications:

ChIP, ELISA, IHC-P, WB

Host Species:

Rabbit

Species Reactivity:

Human, Mouse, Rat

Conjugate:

Unconjugated

Specifications

Host Species:

Rabbit

Species Reactivity:

Human, Mouse, Rat

Immunogen:

Synthetic peptide. This information is considered to be commercially sensitive.

Conjugate:

Unconjugated

Tested Applications:

ChIP, ELISA, IHC-P, WB

Application Note:

WB,1:500 - 1:2000, IHC-P,1:50 - 1:200, ChIP,5μg antibody for 10μg-15μg of Chromatin, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Positive Control 1:

U-2 OS,A-431,HT-1080,Mouse brain,Mouse spleen,Rat brain

Predicted Molecular Weight:

50kDa

Advanced Validation

Properties

purification:

Affinity purification

Clonality:

Monoclonal

Isotype:

IgG

physical-state:

Liquid

Buffer:

Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.

Concentration:

Batch dependent

Storage Conditions:

Store at -20℃. Avoid freeze / thaw cycles.

Additional Info

Ncbi Official Symbol:

RUVBL1

Additional Names:

RVB1; TIH1; ECP54; TIP49; ECP-54; INO80H; NMP238; PONTIN; TIP49A; NMP 238; Pontin52; RUVBL1/TIP49A/PONTIN

Protein Accession Number:

Q9Y265

Ncbi Gene Id Number:

8607

User Note:

Optimal dilutions for each application to be determined by the researcher.

Background

Background:

This gene encodes a protein that has both DNA-dependent ATPase and DNA helicase activities and belongs to the ATPases associated with diverse cellular activities (AAA+) protein family. The encoded protein associates with several multisubunit transcriptional complexes and with protein complexes involved in both ATP-dependent remodeling and histone modification. Alternate splicing results in multiple transcript variants.

Citations

Set Contents

Disclaimer

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:

This product is for research use only.

CATALOG NUMBER:

84-122

List Size:

100 uL

List Price:

$477.00

Datasheet

Shipping Info

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$35 Dry Ice Shipment fee may be required on some items.

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RUVBL1/TIP49A/PONTIN Rabbit Recombinant Antibody (84-122)

Clonality:

Tested Applications:

Host Species:

Species Reactivity:

Conjugate:

Host Species:

Species Reactivity:

Immunogen:

Conjugate:

Tested Applications:

Application Note:

Positive Control 1:

Predicted Molecular Weight:

purification:

Clonality:

Isotype:

physical-state:

Buffer:

Concentration:

Storage Conditions:

Ncbi Official Symbol:

Additional Names:

Protein Accession Number:

Ncbi Gene Id Number:

User Note:

Background:

Disclaimer:

List Size:

Shipping Info

Customize your order

Custom Antibody Services

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