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Western blot analysis of various lysates using Actin (plant specific) Mouse mAb at 1:5000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 30 s.
Western blot analysis of lysates from Arabidopsis thaliana tissue using Actin (plant specific) Mouse mAb at 1:2000-1:20000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST.
Western blot analysis of various lysates using Actin (plant specific) Mouse mAb at 1:5000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 30 s.

Actin (plant specific) Mouse Recombinant Antibody (84-494)

CATALOG NUMBER: 84-494

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Mouse
Species Reactivity:
Various Plants
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Various Plants
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
WB,1:2000 - 1:10000, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Predicted Molecular Weight:
45kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG1
physical-state:
Liquid
Buffer:
Buffer: PBS with 0.02% sodium azide,1% BSA,50% glycerol,pH7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
ACTB
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

84-494

List Size:
100 uL

List Price:

$204.00

Datasheet
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