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Western blot analysis of various lysates using Phospho-CRK/CRKII-Y221 Rabbit mAb at 1:1000 dilution. Both NIH/3T3 cells and C6 cells were treated with Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% BSA. | Detection: ECL Basic Kit. | Exposure time: 10s.
Western blot analysis of various lysates using Phospho-CRK/CRKII-Y221 Rabbit mAb at 1:1000 dilution. Both NIH/3T3 cells and C6 cells were treated with Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% BSA. | Detection: ECL Basic Kit. | Exposure time: 10s.

Phospho-CRK/CRKII-Y221 Rabbit Recombinant Antibody (84-623)

CATALOG NUMBER: 84-623

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
WB,1:500 - 1:1000, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Control 1:
NIH/3T3 treated with Calyculin A,C6 treated with Calyculin A
Predicted Molecular Weight:
34kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
CRK
Additional Names:
p38; CRKII; Phospho-CRK/CRKII-Y221
Protein Accession Number:
P46108
Ncbi Gene Id Number:
1398
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. The product of this gene has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

84-623

List Size:
100 uL

List Price:

$540.00

Datasheet
Shipping Info

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