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Western blot analysis of lysates from PC-12 cells using Phospho-DRP1-S616 Rabbit mAb at 1:1000 dilution incubated at room temperature for 1.5 hours. PC-12 cells were treated with 10% FBS at 37℃ for 30 minutes after serum-starvation overnight. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 30 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 90s.
Western blot analysis of various lysates using Phospho-DRP1-S616 Rabbit mAb at 1:5000 dilution incubated overnight at 4℃. HeLa and NIH/3T3 cells were treated with Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 30 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 20s.
Immunoprecipitation of Phospho-DRP1-S616 from 600 µg extracts of HeLa cells treated with Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight was performed using 1 µg of Phospho-DRP1-S616 Rabbit mAb. Rabbit Control IgG was used to precipitate the Control IgG sample. IP samples were eluted with 1X Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using Phospho-DRP1-S616 Rabbit mAb at a dilution of 1:5000.
Western blot analysis of lysates from PC-12 cells using Phospho-DRP1-S616 Rabbit mAb at 1:1000 dilution incubated at room temperature for 1.5 hours. PC-12 cells were treated with 10% FBS at 37℃ for 30 minutes after serum-starvation overnight. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 30 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 90s.

Phospho-DRP1-S616 Rabbit Recombinant Antibody (84-773)

CATALOG NUMBER: 84-773

Clonality:
Monoclonal
Tested Applications:
ELISA, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IP, WB
Application Note:
WB,1:1000 - 1:10000, IP,0.5μg-4μg antibody for 500μg-700μg extracts of whole cells, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Predicted Molecular Weight:
82kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS with 0.09% Sodium azide,0.05% BSA,50% glycerol,pH7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
DNM1L
Additional Names:
DLP1; DRP1; DVLP; EMPF; OPA5; EMPF1; DYMPLE; HDYNIV
Protein Accession Number:
O00429
Ncbi Gene Id Number:
10059
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the dynamin superfamily of GTPases. The encoded protein mediates mitochondrial and peroxisomal division, and is involved in developmentally regulated apoptosis and programmed necrosis. Dysfunction of this gene is implicated in several neurological disorders, including Alzheimer's disease. Mutations in this gene are associated with the autosomal dominant disorder, encephalopathy, lethal, due to defective mitochondrial and peroxisomal fission (EMPF). Alternative splicing results in multiple transcript variants encoding different isoforms.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

84-773

List Size:
100 uL

List Price:

$540.00

Datasheet
Shipping Info

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