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Western blot analysis of various lysates using GATA3 Rabbit mAb at 1:1000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 10s.
Immunoprecipitation analysis of 300 μg extracts of Jurkat cells using 3 μg GATA3 antibody. Western blot was performed from the immunoprecipitate using GATA3 antibody at a dilution of 1:500.
Chromatin immunoprecipitation analysis of extracts of MCF7 cells, using GATA3 Rabbit mAb antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Chromatin immunoprecipitations were performed with cross-linked chromatin from 293T cells transfected with GATA3 and GATA3. The ChIP sequencing results indicate the enrichment pattern of GATA3 in selected genomic region and representative gene loci (SH3RF3), as shown in figure.
Chromatin immunoprecipitations were performed with cross-linked chromatin from 293T cells transfected with GATA3 and GATA3. The ChIP sequencing results indicate the enrichment pattern of GATA3 in selected genomic region and representative gene loci (SH3RF3), as shown in figure.
Western blot analysis of various lysates using GATA3 Rabbit mAb at 1:1000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 10s.

GATA3 Rabbit Recombinant Antibody (81-545)

CATALOG NUMBER: 81-545

Clonality:
Monoclonal
Tested Applications:
ChIP, ChIP-seq, ELISA, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ChIP, ChIP-seq, ELISA, IP, WB
Application Note:
WB,1:1000 - 1:4000, IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells, ChIP,5μg antibody for 10μg-15μg of Chromatin, ChIP-seq,1:50 - 1:100, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Control 1:
SH-SY5Y,MCF7
Predicted Molecular Weight:
48kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
GATA3
Additional Names:
HDR; HDRS; GATA3
Protein Accession Number:
P23771
Ncbi Gene Id Number:
2625
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a protein which belongs to the GATA family of transcription factors. The protein contains two GATA-type zinc fingers and is an important regulator of T-cell development and plays an important role in endothelial cell biology. Defects in this gene are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

81-545

List Size:
100 uL

List Price:

$540.00

Datasheet
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