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Western blot analysis of lysates from wild type (WT) and 293F cells transfected with DARPP32, using DARPP32 Rabbit mAb at 1:2000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 0.5s.
Western blot analysis of lysates from Mouse brain, using DARPP32 Rabbit mAb at 1:2000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 30s.
Immunofluorescence analysis of 293F-PPP1R1B and 293F cells using DARPP32 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Confocal imaging of frozen sections Mouse brain tissue using DARPP32 Rabbit mAb followed by a further incubation with Cy3-conjugated Goat Anti-Rabbit IgG (H+L) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x.
Confocal imaging of frozen sections Rat brain tissue using DARPP32 Rabbit mAb followed by a further incubation with Cy3-conjugated Goat Anti-Rabbit IgG (H+L) (Red). DAPI was used for nuclear staining (Blue). antigen Objective: 40x.
Western blot analysis of lysates from wild type (WT) and 293F cells transfected with DARPP32, using DARPP32 Rabbit mAb at 1:2000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 0.5s.

DARPP32 Rabbit Recombinant Antibody (82-550)

CATALOG NUMBER: 82-550

Clonality:
Monoclonal
Tested Applications:
ELISA, IF-F, IF/ICC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF-F, IF/ICC, WB
Application Note:
WB,1:1000 - 1:5000, IF/ICC,1:50 - 1:200, IF-F,1:50 - 1:200, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Control 1:
293F transfected with DARPP32, Mouse brain
Predicted Molecular Weight:
23 kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
PPP1R1B
Additional Names:
DARPP32; DARPP-32
Protein Accession Number:
Q9UD71
Ncbi Gene Id Number:
84152
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a bifunctional signal transduction molecule. Dopaminergic and glutamatergic receptor stimulation regulates its phosphorylation and function as a kinase or phosphatase inhibitor. As a target for dopamine, this gene may serve as a therapeutic target for neurologic and psychiatric disorders. Multiple transcript variants encoding different isoforms have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

82-550

List Size:
100 uL

List Price:

$477.00

Datasheet
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