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Western blot analysis of lysates from wild type (WT) and CHO cells transfected with CD278 using CD278 Rabbit mAb at 1:1000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Enhanced Kit. | Exposure time: 180s.
Confocal imaging of CHO (Transfection,left) and CHO cells (negative control,right) using CD278 Rabbit mAb(Red). DAPI was used for nuclear staining (blue). Objective: 100x.
Flow cytometry: 1X10^6 CHO cells (negative control,left) and CHO (Transfection,right) were surface-stained with CD278 Rabbit mAb(2 μg/mL,orange line) or PSFlo 594 Rabbit IgG isotype control,followed by ABflo™ 594-conjugated Goat Anti-Rabbit IgG (H+L) (1:500 dilution) staining.Non-fluorescently stained cells were used as blank control (red line).
Flow cytometry:1X10^6 CHO(Transfection) cells were surface-stained with PSFlo 594 Rabbit IgG isotype control or CD278 Rabbit mAb(2 μg/mL,right).
Western blot analysis of lysates from wild type (WT) and CHO cells transfected with CD278 using CD278 Rabbit mAb at 1:1000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Enhanced Kit. | Exposure time: 180s.

ICOS/CD278 Rabbit Recombinant Antibody (82-824)

CATALOG NUMBER: 82-824

Clonality:
Monoclonal
Tested Applications:
ELISA, FC, IF/ICC, WB
Host Species:
Rabbit
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, FC, IF/ICC, WB
Application Note:
WB,1:500 - 1:1000, IF/ICC,1:200 - 1:400, FC,1:500 - 1:1000, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Control 1:
CHO transfected with CD278（Mouse）
Predicted Molecular Weight:
22kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
Icos/CD278
Additional Names:
H4; CCLP; AILIM; CRP-1; Ly115; ICOS/CD278
Protein Accession Number:
Q9WVS0
Ncbi Gene Id Number:
54167
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Predicted to be involved in T cell costimulation; T cell tolerance induction; and cell-cell adhesion. Located in external side of plasma membrane. Is integral component of plasma membrane. Used to study common variable immunodeficiency. Human ortholog(s) of this gene implicated in celiac disease; common variable immunodeficiency; and immunoglobulin alpha deficiency. Orthologous to human ICOS (inducible T cell costimulator).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

82-824

List Size:
100 uL

List Price:

$477.00

Datasheet
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