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Western blot analysis of various lysates using CD74 Rabbit mAb at 1:200000 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Negative control (NC): Jurkat | Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded Human appendix tissue using CD74 Rabbit mAb at a dilution of 1:40000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using CD74 Rabbit mAb at a dilution of 1:40000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using CD74 Rabbit mAb at a dilution of 1:40000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
Confocal imaging of Raji cells using CD74 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.
Flow cytometry:1X10^6 Jurkat cells (negative control,left) and Raji cells (right) were surface-stained with CD74 Rabbit mAb or PE Rabbit IgG isotype control (5 μl/Test,blue line), followed by PE Donkey anti-rabbit Antibody staining. Non-fluorescently stained cells were used as blank control (red line).
Flow cytometry: 1X10^6 Raji cells were surface-stained with PE Rabbit IgG isotype control (5 μl/Test,left) or CD74 Rabbit mAb.
Western blot analysis of various lysates using CD74 Rabbit mAb at 1:200000 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Negative control (NC): Jurkat | Exposure time: 10s.

CD74 Rabbit Recombinant Antibody (82-851)

CATALOG NUMBER: 82-851

Clonality:
Monoclonal
Tested Applications:
ELISA, FC, IF/ICC, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Tested Applications:
ELISA, FC, IF/ICC, IHC-P, WB
Application Note:
WB,1:100000 - 1:400000, IHC-P,1:10000 - 1:50000, IF/ICC,1:200 - 1:800, FC,1:500 - 1:1000, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Predicted Molecular Weight:
18kDa/24kDa/26kDa/31kDa/34kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
CD74
Additional Names:
II; p33; CLIP; DHLAG; HLADG; Ia-GAMMA
Protein Accession Number:
P04233-2
Ncbi Gene Id Number:
972
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene associates with class II major histocompatibility complex (MHC) and is an important chaperone that regulates antigen presentation for immune response. It also serves as cell surface receptor for the cytokine macrophage migration inhibitory factor (MIF) which, when bound to the encoded protein, initiates survival pathways and cell proliferation. This protein also interacts with amyloid precursor protein (APP) and suppresses the production of amyloid beta (Abeta). Multiple alternatively spliced transcript variants encoding different isoforms have been identified.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

82-851

List Size:
100 uL

List Price:

$540.00

Datasheet
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