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Western blot analysis of lysates from wild type (WT) and 293F cells transfected with ENPP3/CD203c using ENPP3/CD203c Rabbit mAb at 1:1000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 30 s.
Western blot analysis of various lysates using ENPP3/CD203c Rabbit mAb at 1:1000 dilution incubated at room temperature for 1.5 hours. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Negative control (NC): Daudi. | Exposure time: 60 s.
Confocal imaging of KU812 cells and Daudi cells (negative) using ENPP3/CD203c Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.
Confocal imaging of 293F cells transfected with ENPP3 using ENPP3/CD203c followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.
Flow cytometry: 1X10^6 293F cells (negative control,left) and 293F (Transfection,right) cells were surface-stained with ENPP3/CD203c Rabbit mAb or PSFlo 647 Rabbit IgG isotype control, followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).
Flow cytometry: 1X10^6 293F (Transfection) cells were surface-stained with PSFlo 647 Rabbit IgG isotype control or ENPP3/CD203c Rabbit mAb.
Western blot analysis of lysates from wild type (WT) and 293F cells transfected with ENPP3/CD203c using ENPP3/CD203c Rabbit mAb at 1:1000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 30 s.

ENPP3/CD203c Rabbit Recombinant Antibody (82-972)

CATALOG NUMBER: 82-972

Clonality:
Monoclonal
Tested Applications:
ELISA, FC, IF/ICC, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, FC, IF/ICC, WB
Application Note:
WB,1:1000 - 1:2000, IF/ICC,1:200 - 1:800, FC,1:500 - 1:1000, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Predicted Molecular Weight:
100kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
ENPP3
Additional Names:
B10; NPP3; PDNP3; CD203c; PD-IBETA; ENPP3/CD203c
Protein Accession Number:
O14638
Ncbi Gene Id Number:
5169
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene belongs to a series of ectoenzymes that are involved in hydrolysis of extracellular nucleotides. These ectoenzymes possess ATPase and ATP pyrophosphatase activities and are type II transmembrane proteins. Expression of the related rat mRNA has been found in a subset of immature glial cells and in the alimentary tract. The corresponding rat protein has been detected in the pancreas, small intestine, colon, and liver. The human mRNA is expressed in glioma cells, prostate, and uterus. Expression of the human protein has been detected in uterus, basophils, and mast cells. Two transcript variants, one protein coding and the other non-protein coding, have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

82-972

List Size:
100 uL

List Price:

$477.00

Datasheet
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