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Western blot analysis of various lysates using CD24 Rabbit mAb at 1:1000 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 20 s.
Confocal imaging of Neuro-2a cells using CD24 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (Red). The cells were counterstained with α-Tubulin Mouse mAb followed by incubation with PSFlo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
Confocal imaging of paraffin-embedded Mouse speeln tissue using CD24 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
Flow cytometry: 1X10^6 RAW 264.7 cells (Low Expression,left) and C57BL/6 mouse Splenocytes (right) were surface-stained with CD24 Rabbit mAb or PSFlo 488 Rabbit IgG isotype control, followed by FITC conjugated goat anti-Rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).
Flow cytometry: 1X10^6 C57BL/6 mouse Splenocytes cells were surface-stained with PSFlo 488 Rabbit IgG isotype control or CD24 Rabbit mAb.
Western blot analysis of various lysates using CD24 Rabbit mAb at 1:1000 dilution incubated overnight at 4℃. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 20 s.

CD24 Rabbit Recombinant Antibody (83-098)

CATALOG NUMBER: 83-098

Clonality:
Monoclonal
Tested Applications:
ELISA, FC, IF-P, IF/ICC, WB
Host Species:
Rabbit
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, FC, IF-P, IF/ICC, WB
Application Note:
WB,1:1000 - 1:4000, IF/ICC,1:200 - 1:2000, IF-P,1:200 - 1:2000, FC,1:500 - 1:1000, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Predicted Molecular Weight:
8kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
Cd24
Additional Names:
HSA; Cd24; Ly-52; nectadrin
Protein Accession Number:
P24807
Ncbi Gene Id Number:
12484
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CD24, also know as heat stable antigen HSA, is a P-selectin ligand involved in adhesion. It is a GPI-anchored glycoprotein expressed on many types of cells, including hematopoietic cells, neural cells, and epithelial cells. CD24 is widely used to delineate stages of lymphocyte development . It also binds to Siglec-10 in humans or Siglec-G in mice . CD24 is frequently used as a marker to identify and isolate cancer stem cells in various cancer types .

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

83-098

List Size:
100 uL

List Price:

$477.00

Datasheet
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