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Western blot analysis of various lysates using T-bet/Tbx21 Rabbit mAb at 1:1500 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Negative control (NC): HeLa. | Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded Human liver (negative control sample) tissue using T-bet/Tbx21 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human peripheral T cell lymphoma tissue using T-bet/Tbx21 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using T-bet/Tbx21 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Flow cytometry: 1X10^6 HeLa cells (negative control,left) and NK-92 cells (right) were intracellularly-stained with T-bet/Tbx21 Rabbit mAb or Rabbit IgG isotype control, followed by FITC conjugated goat anti-Rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).
Flow cytometry: 1X10^6 NK-92 cells were intracellularly-stained with Rabbit IgG isotype control or T-bet/Tbx21 Rabbit mAb, followed by FITC conjugated goat anti-Rabbit pAb staining.
Western blot analysis of various lysates using T-bet/Tbx21 Rabbit mAb at 1:1500 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25 μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Negative control (NC): HeLa. | Exposure time: 30s.

T-bet/Tbx21 Rabbit Recombinant Antibody (83-133)

CATALOG NUMBER: 83-133

Clonality:
Monoclonal
Tested Applications:
ELISA, FC (intra), IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, FC (intra), IHC-P, WB
Application Note:
WB,1:1000 - 1:5000, IHC-P,1:200 - 1:800, FC (intra),1:500 - 1:1000, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Predicted Molecular Weight:
58kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
TBX21
Additional Names:
TBX21; T-PET; T-bet; TBET; TBLYM; T-box 21
Protein Accession Number:
Q9UL17
Ncbi Gene Id Number:
30009
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene is the human ortholog of mouse Tbx21/Tbet gene. Studies in mouse show that Tbx21 protein is a Th1 cell-specific transcription factor that controls the expression of the hallmark Th1 cytokine, interferon-gamma (IFNG). Expression of the human ortholog also correlates with IFNG expression in Th1 and natural killer cells, suggesting a role for this gene in initiating Th1 lineage development from naive Th precursor cells.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

83-133

List Size:
100 uL

List Price:

$477.00

Datasheet
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