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Recombinant Human IL-10 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Recombinant Human IL-10 promote cell proliferation of MC/9 2 mouse mast cells. The ED<sub>50</sub> for this effect is 0.13-0.52 ng/mL, corresponding to a specific activity of 1.92×10<sup>6</sup>-7.69×10<sup>6</sup>units/mg.
Immobilized Human IL10 (Catalog: 84-878) at 2 μg/mL (100 μL/well) can bind Human IL10RA (Catalog: 85-674) with a linear range of 0.1-2.9 ng/mL.
Recombinant Human IL-10 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

Human IL-10 Recombinant Protein (84-878)

CATALOG NUMBER: 84-878

Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Specifications
source-species:
HEK293 cells
Species:
Human
source-species:
HEK293 cells
Recombinant Protein Sequence:
Ser19-Asn178
Fusion Tag:
C-His
Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Application Note:
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity Test
Predicted Molecular Weight:
19.49 kDa
Biological Activity:
1.Measured in a cell proliferation assay using MC/9-2 mouse mast cells. The ED50 for this effect is 0.13-0.52 ng/mL, corresponding to a specific activity of 1.92×106-7.69×106units/mg.
2.Measured by its binding ability in a functional ELISA.Immobilized Human IL10 at 2 μg/mL (100 μL/well) can bind Human IL10RA with a linear range of 0.1-2.9 ng/mL.
Purity:
≥ 90 % as determined by SDS-PAGE.
Endotoxin:
< 0.1 EU/μg of the protein by LAL method.
physical-state:
Lyophilized
Buffer:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Storage Conditions:
Store at -20°C.Store the lyophilized protein at -20°C to -80 °C up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Additional Names:
CSIF, GVHDS, IL-10, IL10A, TGIF,IL10,GVHDS,IL-10,IL10A,TGIF
Protein Accession Number:
NP_000563.1
Ncbi Gene Id Number:
3586
Background:
The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.

FOR RESEARCH USE ONLY

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Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

84-878

List Size:
0.01 mg, 0.05 mg

List Price:

Price range: $140.00 through $350.00

Datasheet
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