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Recombinant Human TNFRSF12A/TWEAKR/CD266 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
The purity of Human TNFRSF12A/FN14/TWEAKR Protein (Cat.84-949) was greater than 90% as determined by SEC-HPLC.
Immobilized Human TNFSF12 at 2 μg/mL (100 μL/well) can bind Human TNFRSF12A with a linear range of 0.1-2.3 ng/mL.
Recombinant Human TNFRSF12A inhibits the TWEAK-dependent proliferation of HUVEC human umbilical vein endothelial cells. The ED<sub>50</sub> for this effect is 20-80 ng/mL in the presence of 15 ng/mL recombinant human TWEAK.
Recombinant Human TNFRSF12A/TWEAKR/CD266 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

Human TNFRSF12A/TWEAKR/CD266 Recombinant Protein (84-949)

CATALOG NUMBER: 84-949

Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Specifications
source-species:
HEK293 cells
Species:
Human
source-species:
HEK293 cells
Recombinant Protein Sequence:
Glu28-Trp79
Fusion Tag:
C-hFc&His
Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Application Note:
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity Test
Predicted Molecular Weight:
32.30 kDa
Biological Activity:
1.Measured by its ability to inhibit TWEAK-induced apoptosis in HT-29 human colon adenocarcinoma cells. The ED50 for this effect is 2-12 μg/mL in the presence of 1 μg/mL recombinant human TWEAK.
2.Measured by its binding ability in a functional ELISA. Immobilized Human TNFSF12 at 2 μg/mL (100 μL/well) can bind Human TNFRSF12A with a linear range of 0.1-2.3 ng/mL.
3.Measured by its ability to inhibit the TWEAK-dependent proliferation of HUVEC human umbilical vein endothelial cells. The ED50 for this effect is 20-80 ng/mL in the presence of 15 ng/mL recombinant human TWEAK.
Purity:
≥ 90 % as determined by SDS-PAGE;≥ 90 % as determined by HPLC.
Endotoxin:
< 0.1 EU/μg of the protein by LAL method.
physical-state:
Lyophilized
Buffer:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Storage Conditions:
Store at -20°C.Store the lyophilized protein at -20°C to -80 °C up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Additional Names:
CD266, FN14, TWEAKR,TNFRSF12A,FN14,TWEAKR
Protein Accession Number:
NP_057723.1
Ncbi Gene Id Number:
51330
Background:
Fn14 (tumor necrosis factor receptor superfamily, member 12A), also known as TNFRSF12A, is the receptor for TNFSF12/TWEAK. Human and mouse TNFRSF12A share 82% aa sequence identity. TNFRSF12A transcript was expressed at high levels in heart, placenta, and kidney, at intermediate levels in lung, skeletal muscle, and pancreas, and at low levels in brain and liver. In addition, elevated TNFRSF12A expression was found in human liver cancer cell lines and hepatocellular carcinoma specimens. TNFRSF12A is the weak inducer of apoptosis in some cell types. It promotes angiogenesis and the proliferation of endothelial cells. TNFRSF12A may modulate cellular adhesion to matrix proteins.

FOR RESEARCH USE ONLY

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Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

84-949

List Size:
0.05 mg

List Price:

$280.00

Datasheet
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