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Recombinant Human IL-6 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Immobilized Human IL6R at 1 μg/mL (100 μL/well) can bind Human IL-6 with a linear range of 2-15 ng/mL.
Measured in a cell proliferation assay using TF-1 Human erythroleukemic cells. The ED<sub>50</sub> for this effect is 0.10-0.40 ng/mL. The specific activity of Recombinant Human IL-6 is >1.2 × 10<sup>8</sup> IU/mg, which is calibrated against the human IL-6 WHO International Standard (NIBSC code: 21/308).
Recombinant Human IL-6 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

Human IL-6 Recombinant Protein (84-983)

CATALOG NUMBER: 84-983

Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Specifications
source-species:
HEK293 cells
Species:
Human
source-species:
HEK293 cells
Recombinant Protein Sequence:
Val30-Met212
Fusion Tag:
C-His
Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Application Note:
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity Test
Predicted Molecular Weight:
21.65 kDa
Biological Activity:
1.Measured by its binding ability in a functional ELISA.Immobilized Human IL6R at 1 μg/mL (100 μL/well) can bind Human IL-6 with a linear range of 2-15 ng/mL.
2.Measured in a cell proliferation assay using TF-1 Human erythroleukemic cells. The ED50 for this effect is 0.10-0.40 ng/mL. The specific activity of Recombinant Human IL-6 is >1.2 × 108 IU/mg, which is calibrated against the human IL-6 WHO International Standard (NIBSC code: 21/308).
Purity:
≥ 95 % as determined by SDS-PAGE.
Endotoxin:
< 0.1 EU/μg of the protein by LAL method.
physical-state:
Lyophilized
Buffer:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Storage Conditions:
Store at -20°C.Store the lyophilized protein at -20°C to -80 °C up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Additional Names:
IL6,BSF-2,BSF2,CDF,HGF,HSF,IFN-beta-2,IFNB2,IL-6
Protein Accession Number:
NP_000591.1
Ncbi Gene Id Number:
3569
Background:
Interleukin-6 (IL-6) is a multifunctional α-helical cytokine that regulates cell growth and differentiation of various tissues, which is known particularly for its role in the immune response and acute phase reactions. The encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this protein is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

84-983

List Size:
0.01 mg, 0.05 mg

List Price:

Price range: $112.00 through $252.00

Datasheet
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