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Recombinant Mouse CSF-3/G-CSF Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Recombinant Mouse G-CSF/CSF3 stimulates cell proliferation of the NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED<sub>50</sub> for this effect is 38.7-154.8 pg/mL, corresponding to a specific activity of 6.46×10<sup>6</sup>-2.58×10<sup>7</sup> units/mg.
Recombinant Mouse CSF-3/G-CSF Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

Mouse CSF-3/G-CSF Recombinant Protein (85-100)

CATALOG NUMBER: 85-100

Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Specifications
source-species:
HEK293 cells
Species:
Mouse
source-species:
HEK293 cells
Recombinant Protein Sequence:
Met1-Ala208
Fusion Tag:
C-His
Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Application Note:
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity Test
Predicted Molecular Weight:
19.79 kDa
Biological Activity:
Measured in a cell proliferation assay using NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is 38.7-154.8 pg/mL, corresponding to a specific activity of 6.46×106-2.58×107 units/mg.
Purity:
≥ 95 % as determined by SDS-PAGE.
Endotoxin:
< 1 EU/μg of the protein by LAL method.
physical-state:
Lyophilized
Buffer:
Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.Contact us for customized product form or formulation.
Storage Conditions:
Store at -20°C.Store the lyophilized protein at -20°C to -80 °C up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Additional Names:
Granulocyte colony-stimulating factor;Csf3;G-CSF,GCSF
Protein Accession Number:
P09920
Ncbi Gene Id Number:
12985
Background:
Granulocyte colony-stimulating factor (G-CSF) is a growth factor and an essential cytokine which belongs to theIL-6 superfamily. Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesisby controlling the production, differentiation, and function of 2 related white cell populations of the blood, thegranulocytes and the monocytes-macrophages. G-CSF binding to its receptor G-CSF-R which belongs to thecytokine receptor type I family depends on the interaction of alpha-helical motifs of the former and twofibronectin type III as well as an immunoglobulin-like domain of the latter. G-CSF is a cytokine that have beendemonstrated to improve cardiac function and perfusion in myocardial infarction. And it was initially evaluatedas a stem cell mobilizer and erythropoietin as a cytoprotective agent.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

85-100

List Size:
0.01 mg, 0.05 mg

List Price:

Price range: $140.00 through $350.00

Datasheet
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