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Recombinant Human TNF-alpha Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Recombinant Human TNF-alpha induces cytotoxicity in the L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED<sub>50</sub> for this effect is 0.90-3.60 ng/mL, corresponding to a specific activity of 2.78×10<sup>5</sup>~1.11×10<sup>6</sup> units/mg.
At concentrations ranging from 0.01 to 5 ng/mL, M5 factor (86-065 IL1A,84-993 IL17A,85-654 IL22,85-170 TNFα,84-841 OSM)promotes the proliferation of HaCaT cells.
Recombinant Human TNF-alpha Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

Human TNF-alpha Recombinant Protein (85-170)

CATALOG NUMBER: 85-170

Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Specifications
source-species:
HEK293 cells
Species:
Human
source-species:
HEK293 cells
Recombinant Protein Sequence:
Val 77 - Leu 233
Fusion Tag:
C-His
Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Application Note:
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity Test
Predicted Molecular Weight:
18.19 kDa
Biological Activity:
1.Recombinant Human TNF-alpha induces cytotoxicity in the L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 0.90-3.60 ng/mL, corresponding to a specific activity of 2.78×105~1.11×106 units/mg.2.At concentrations ranging from 0.01 to 5 ng/mL, M5 factor (RP03505 IL1A,RP00212 IL17A,RP01616 IL22,RP00993 TNFα,RP00054 OSM)promotes the proliferation of HaCaT cells.
Purity:
≥ 95 % as determined by SDS-PAGE.
Endotoxin:
< 0.1 EU/μg of the protein by LAL method.
physical-state:
Lyophilized
Buffer:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Storage Conditions:
Store at -20°C.Store the lyophilized protein at -20°C to -80 °C up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Additional Names:
TNF, DIF, TNF-alpha, TNFA, TNFSF2, TNLG1F, tumor necrosis factor,TNF-α,DIF,TNF-alpha,TNFA,TNFSF2,TNLG1F,TNF alpha
Protein Accession Number:
NP_000585.2
Ncbi Gene Id Number:
7124
Background:
Tumor necrosis factor alpha (TNF-alpha), also kNown as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. KNockout studies in mice also suggested the neuroprotective function of this cytokine.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

85-170

List Size:
0.02 mg, 0.05 mg

List Price:

Price range: $126.00 through $168.00

Datasheet
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