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Recombinant Human IFN-gamma Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
The purity of Recombinant Human IFN-gamma Protein is greater than 95% as determined by SEC-HPLC.
Immobilized Human FNGR1/CD119 at 1 μg/mL (100 μL/well) can bind Human IFNG with a linear range of 0.98-1.97ng/mL.
Recombinant Human IFN-gamma induces cytotoxicity in the HT-29 cells. The ED<sub>50</sub> for this effect is 0.74-2.96 ng/mL, corresponding to a specific activity of 3.38×10<sup>5</sup>~1.35×10<sup>6</sup> units/mg.
Flow cytometry: 1X10<sup>6</sup> A549 cells treated with IFN-γ (85-195,1 ng/mL) were surface-stained with PE Rabbit IgG isotype control (left) or PE Rabbit anti-Human PD-L1/CD274 mAb (right)
Flow cytometry: 1X10<sup>6</sup> NCI-H460 cells treated with IFN-γ (85-195,1 ng/mL) were surface-stained with PE Rabbit IgG isotype control (left) or PE Rabbit anti-Human PD-L1/CD274 mAb (right)
Recombinant Human IFN-gamma Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

Human IFN-gamma Recombinant Protein (85-195)

CATALOG NUMBER: 85-195

Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Specifications
Source Species:
E. coli
Species:
Human
Source Species:
E. coli
Recombinant Protein Sequence:
Gln24-Gln166
Fusion Tag:
No tag
Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Application Note:
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity Test
Predicted Molecular Weight:
16.78 kDa
Biological Activity:
1.Measured by its binding ability in a functional ELISA. Immobilized Human FNGR1/CD119 at 1 μg/mL (100 μL/well) can bind Human IFNG with a linear range of 0.98-1.97 ng/mL.
2.Measured in a cytotoxicity assay using HT-29 cells. The ED50 for this effect is 0.74-2.96 ng/mL, corresponding to a specific activity of 3.38×105~1.35×106 units/mg.
3.Flow cytometry: 1X106 A549 cells treated with IFN-γ (1 ng/mL) were surface-stained with PE Rabbit IgG isotype control (5 μl/Test,left) or PE Rabbit anti-Human PD-L1/CD274 mAb (5 μl/Test,right).
4.Flow cytometry: 1X106 NCI-H460 cells treated with IFN-γ (1 ng/mL) were surface-stained with PE Rabbit IgG isotype control (5 μl/Test,left) or PE Rabbit anti-Human PD-L1/CD274 mAb (5 μl/Test,right)
Purity:
≥ 95 % as determined by SDS-PAGE;≥ 95 % as determined by HPLC.
Endotoxin:
< 0.1 EU/μg of the protein by LAL method.
Physical State:
Lyophilized
Buffer:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Storage Conditions:
Store at -20°C.Store the lyophilized protein at -20°C to -80 °C up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Additional Names:
IFNG,IFG,IFI
Protein Accession Number:
NP_000610.2
Ncbi Gene Id Number:
3458
Background:
Interferon-gamma (IFN-gamma ) is a secreted protein which belongs to the type II interferon family.Recombinant Human IFN-gamma is a 16.8 kDa protein containing 143 amino acid residues. IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. Interferon-gamma is a central regulator of the immune response and signals via the Janus Activated Kinase (JAK)-Signal Transducer and Activator of Transcription (STAT) pathway.

FOR RESEARCH USE ONLY

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Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

85-195

List Size:
0.02 mg, 0.05 mg

List Price:

Price range: $126.00 through $168.00

Datasheet
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