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Recombinant Human FGF-10 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Recombinant Human FGF-10 Protein promotes proliferation assay using 4MBr-5 rhesus monkey epithelial cells. The ED<sub>50</sub> for this effect is 2.08-8.32 ng/mL, corresponding to a specific activity of 1.20×10<sup>5</sup>~4.81×10<sup>5</sup> units/mg.
Human stomach organoids were cultured with EGF(Cat. 86-064), FGF10(Cat. 85-242), NOG(Cat. 85-332), RSPO1(Cat. 84-857), WNT-3a.
Human liver organoids were cultured with EGF(Cat. 86-064), HGF(Cat. 85-644), FGF2(Cat. 85-199), FGF10(Cat. 85-242), NOG(Cat. 85-332), RSPO1(Cat. 84-857), WNT-3a.
Human kidney organoids were cultured with EGF(Cat. 86-064), FGF2(Cat. 85-199), FGF7(Cat. 85-732), FGF9(Cat. 85-725), FGF10(Cat. 85-242), IGF-(Cat. 85-173), NOG(Cat. 85-332), RSPO1(Cat. 84-857), WNT-3a. And further, DKK-1(85-404) was used to induce the establishment of cell polarity.
Human colorectal cancer organoid were cultured with EGF(Cat. 86-064), NOG(Cat. 85-332), FGF10(Cat. 85-242), RSPO1(Cat. 84-857), HGF(Cat. 85-644).
Mouse lung organoids were cultured in Matrigel with medium supplemented with EGF (Cat. 86-064), Noggin (Cat. 85-332), RSPO1 (Cat. 84-857), Wnt-3a, and FGF-10 (Cat. 85-242).
Recombinant Human FGF-10 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

Human FGF-10 Recombinant Protein (85-242)

CATALOG NUMBER: 85-242

Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Specifications
Source Species:
E. coli
Species:
Human
Source Species:
E. coli
Recombinant Protein Sequence:
Gln38-Ser208
Fusion Tag:
No tag
Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Application Note:
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity Test
Predicted Molecular Weight:
19.32 kDa
Biological Activity:
1.Measured in a cell proliferation assay using 4MBr-5 rhesus monkey epithelial cells. The ED50 for this effect is 2.08-8.32 ng/mL, corresponding to a specific activity of 1.20×105~4.81×105 units/mg.
2.Human stomach organoids were cultured with EGF, FGF10, NOG, RSPO1, WNT-3a.
3.Human liver organoids were cultured with EGF, HGF, FGF2, FGF10, NOG, RSPO1, WNT-3a.
4.Human kidney organoids were cultured with EGF, FGF2, FGF7, FGF9, FGF10, IGF-, NOG, RSPO1, WNT-3a. And further, DKK-1 was used to induce the establishment of cell polarity.
Purity:
≥ 95 % as determined by SDS-PAGE;≥ 95 % as determined by HPLC.
Endotoxin:
< 0.1 EU/μg of the protein by LAL method.
Physical State:
Lyophilized
Buffer:
Lyophilized from a 0.22 μm filtered solution of PBS pH7.4.
Storage Conditions:
Store at -20°C.Store the lyophilized protein at -20°C to -80 °C up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Additional Names:
Fibroblast growth factor 10, FGF-10, Keratinocyte growth factor 2,FGF10,KGF2
Protein Accession Number:
NP_004456.1
Ncbi Gene Id Number:
2255
Background:
This protein is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein exhibits mitogenic activity for keratinizing epidermal cells, but essentially no activity for fibroblasts, which is similar to the biological activity of FGF7. Studies of the mouse homolog of suggested that this gene is required for embryonic epidermal morphogenesis including brain development, lung morphogenesis, and initiation of lim bud formation. This gene is also implicated to be a primary factor in the process of wound healing.

FOR RESEARCH USE ONLY

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Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

85-242

List Size:
0.01 mg, 0.05 mg

List Price:

Price range: $140.00 through $350.00

Datasheet
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