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Recombinant Human/Mouse/Rat BDNF Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
The purity of Recombinant Human/Mouse/Rat BDNF Protein (Cat.85-338) was greater than 95% as determined by SEC-HPLC.
Immobilized Recombinant Human/Mouse/Rat BDNF Protein at 5μg/mL (100 μL/well) can bind Recombinant Human TrkB with a linear range of 0.38-1.14 μg/mL.
Immobilized Recombinant Human/Mouse/Rat BDNF Protein at 1μg/mL (100 μL/well) can bind BDNF Rabbit mAb with a linear range of 0.4-2.15ng/mL.
Immobilized Recombinant Human TrkB at 2μg/mL (100 μL/well) can bind Recombinant Human BDNF with a linear range of 1.95-258ng/mL.
Recombinant Human/Mouse/Rat BDNF Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

Human/Mouse/Rat BDNF Recombinant Protein (85-338)

CATALOG NUMBER: 85-338

Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Specifications
source-species:
E. coli
Species:
Human,Mouse,Rat
source-species:
E. coli
Recombinant Protein Sequence:
His129-Arg247
Fusion Tag:
C-His
Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Application Note:
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity Test
Predicted Molecular Weight:
14.35 kDa
Biological Activity:
1.Immobilized Recombinant Human/Mouse/Rat BDNF Protein at 5μg/mL (100 μL/well) can bind Recombinant Human TrkB with a linear range of 0.38-1.14 μg/mL.2.Immobilized Recombinant Human/Mouse/Rat BDNF Protein at 1μg/mL (100 μL/well) can bind BDNF Rabbit mAb with a linear range of 0.4-2.15ng/mL.3.Immobilized Recombinant Human TrkB at 2μg/mL (100 μL/well) can bind Recombinant Human BDNF with a linear range of 1.95-258ng/mL.
Purity:
≥ 95 % as determined by SDS-PAGE;≥95 % as determined by HPLC.
Endotoxin:
< 1 EU/μg of the protein by LAL method.
physical-state:
Lyophilized
Buffer:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Storage Conditions:
Store at -20°C.Store the lyophilized protein at -20°C to -80 °C up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Additional Names:
ANON2, BULN2,BDNF,BULN2
Protein Accession Number:
NP_733929.1.
Ncbi Gene Id Number:
627
Background:
Brain-derived neurotrophic factor (BDNF) is a member of the nerve growth factor family.The neurotrophin family is comprised of at least four proteins including NGF, BDNF, NT-3, and NT-4/5. These secreted cytokines are synthesized as prepropeptides that are proteolytically processed to generate the mature proteins.BDNF cDNA encodes a 247 amino acid residue precursor protein with a signal peptide and a proprotein that are cleaved to yield the 119 amino acid residue mature BDNF. The amino acid sequence of mature BDNF is identical in all mammals examined. BDNF binds with high affinity and specifically activates the TrkB tyrosine kinase receptor .

FOR RESEARCH USE ONLY

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Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

85-338

List Size:
0.01 mg, 0.05 mg

List Price:

Price range: $168.00 through $420.00

Datasheet
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