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Recombinant SARS-CoV Spike S1 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Immobilized at Human ACE2 2μg/mL (100μL/well) can bind SARS-CoV Spike S1 with a linear range of 0.05-36.67 ng/mL.
Recombinant SARS-CoV Spike S1 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

SARS-CoV Spike S1 Recombinant Protein (85-368)

CATALOG NUMBER: 85-368

Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Specifications
source-species:
HEK293 cells
Species:
SARS-CoV
source-species:
HEK293 cells
Recombinant Protein Sequence:
Ser14-Arg667
Fusion Tag:
C-mFc
Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Application Note:
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity Test
Predicted Molecular Weight:
98.50 kDa
Biological Activity:
Measured by its binding ability in a functional ELISA. Immobilized at Human ACE2 2μg/mL (100μL/well) can bind SARS-CoV Spike S1 with a linear range of 0.05-36.67 ng/mL.
Purity:
≥ 90 % as determined by SDS-PAGE.
Endotoxin:
< 1 EU/μg of the protein by LAL method.
physical-state:
Lyophilized
Buffer:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Storage Conditions:
Store at -20°C.Store the lyophilized protein at -20°C to -80 °C up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Additional Names:
Spike,Spike RBD,Spike S1
Protein Accession Number:
NP_828851.1
Ncbi Gene Id Number:
1489668
Background:
The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

85-368

List Size:
0.01 mg, 0.1 mg

List Price:

Price range: $126.00 through $448.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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