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Recombinant Mouse CCL2/MCP-1 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Recombinant Mouse CCL2 inhibit the proliferation of HUVEC(Human Umbilical Vein Endothelial Cells).The ED<sub>50</sub> for this effect is 0.20-0.80 ng/mL.
Recombinant Mouse CCL2 chemoattract THP-1 cells. The ED<sub>50</sub> for this effect is typically 2.87-11.48 ng/mL.
Recombinant Mouse CCL2/MCP-1 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

Mouse CCL2/MCP-1 Recombinant Protein (85-662)

CATALOG NUMBER: 85-662

Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Specifications
source-species:
Pichia
Species:
Mouse
source-species:
Pichia
Recombinant Protein Sequence:
Gln 24-Asn148
Fusion Tag:
No tag
Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Application Note:
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity Test
Predicted Molecular Weight:
13.85 kDa
Biological Activity:
1.Measured by the ability to inhibit the proliferation of HUVEC(Human Umbilical Vein Endothelial Cells).The ED50 for this effect is 0.20-0.80 ng/mL.
2.Measured by its ability to chemoattract THP-1 cells. The ED50 for this effect is typically 2.87-11.48 ng/mL.
Purity:
≥ 95 % as determined by SDS-PAGE.
Endotoxin:
< 0.1 EU/μg of the protein by LAL method.
physical-state:
Lyophilized
Buffer:
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Storage Conditions:
Store at -20°C.Store the lyophilized protein at -20°C to -80 °C up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Additional Names:
C-C motif chemokine ligand 2, CCL2, GDCF-2, HC11, HSMCR30, MCAF, Mcp1, MCP-1, SCYA2, SMC-CF,CCL2
Protein Accession Number:
NP_035463.1
Ncbi Gene Id Number:
20296
Background:
Monocyte chemoattractant protein 1 (MCP-1), also called CCL2, belongs to a group of CC chemokines located in chromosome 17q11.2. MCP-1 protein interacts with chemokine C-C motif receptor 2 (CCR2) to activate and recruit monocytes, macrophages, CD4+ T cells and immature dendritic cells to the site of infection. The presence of MCP-1 protein in an adequate concentration is important for granuloma formation and M. tuberculosis clearance.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

85-662

List Size:
0.02 mg, 0.05 mg

List Price:

Price range: $210.00 through $350.00

Datasheet
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