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Recombinant Mouse FGF-10 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Measured in a cell proliferation assay using 4MBr-5 rhesus monkey epithelial cells. The ED<sub>50</sub> for this effect is 3.66-14.64 ng/mL, corresponding to a specific activity of 56.83×10<sup>4</sup>~2.73×10<sup>5</sup> units/mg.
Recombinant Mouse FGF-10 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

Mouse FGF-10 Recombinant Protein (85-842)

CATALOG NUMBER: 85-842

Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Specifications
source-species:
E. coli
Species:
Mouse
source-species:
E. coli
Recombinant Protein Sequence:
Gln37-Thr209
Fusion Tag:
NO-Tag
Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Application Note:
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity Test
Predicted Molecular Weight:
19.54 kDa
Biological Activity:
Measured in a cell proliferation assay using 4MBr-5 rhesus monkey epithelial cells. The ED50 for this effect is 3.66-14.64 ng/mL, corresponding to a specific activity of 56.83×104~2.73×105 units/mg.
Purity:
≥ 95 % as determined by SDS-PAGE.
Endotoxin:
< 1 EU/μg of the protein by LAL method.
physical-state:
Lyophilized
Buffer:
Lyophilized from a 0.22 μm filtered solution of 20mMPB,800mMNaCl, pH7.4
Storage Conditions:
Store at -20°C.Store the lyophilized protein at -20°C to -80 °C up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Additional Names:
Fibroblast growth factor 10, FGF-10, Keratinocyte growth factor 2,Fgf10
Protein Accession Number:
NP_032028.1
Ncbi Gene Id Number:
14165
Background:
Fibroblast Growth Factor 10 (FGF 10) is an evolutionary conserved secreted growth factor mediating mostly mesenchymal to epithelial signaling. FGF 10 belongs to the FGF 7 subfamily and shares similar biochemical and amino acid sequences with its constituent members (FGF3, FGF 7 and FGF 22). As a paracrine FGF, FGF 10 elicits its biological responses by activating the fibroblast growth factor receptor 2b (FGF R 2b), is crucial for governing proximal distal outgrowth as well as patterning and acts upstream of the known apical ectodermal ridge (AER) marker FGF 8. FGF10 is also implicated in pancreatic cancer, and that overexpression of FGFR2b is associated with metastatic invasion.

FOR RESEARCH USE ONLY

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Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

85-842

List Size:
0.01 mg, 0.05 mg

List Price:

Price range: $168.00 through $420.00

Datasheet
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