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Recombinant Mouse Alkaline phosphatase,tissue-nonspecific isozyme/ALPL Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.
Measured by its ability to cleave a fluorogenic substrate, 4-Methylumbelliferyl phosphate (4-MUP). The specific activity is >51590.2 pmol/min/μg, as measured under the described conditions.
Recombinant Mouse Alkaline phosphatase,tissue-nonspecific isozyme/ALPL Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.

Mouse Alkaline Phosphatase,Tissue-nonspecific Isozyme/ALPL Recombinant Protein (85-959)

CATALOG NUMBER: 85-959

Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Specifications
source-species:
HEK293 cells
Species:
mouse
source-species:
HEK293 cells
Recombinant Protein Sequence:
Phe18-Ala500
Fusion Tag:
C-6His
Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Application Note:
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity Test
Predicted Molecular Weight:
54.26 KDa
Biological Activity:
Measured by its ability to cleave a fluorogenic substrate, 4-Methylumbelliferyl phosphate (4-MUP). The specific activity is >51590.2 pmol/min/μg, as measured under the described conditions.
Purity:
≥ 90 % as determined by SDS-PAGE.
Endotoxin:
< 0.01 EU/μg of the protein by LAL method
physical-state:
Lyophilized
Buffer:
Lyophilized from a 0.2 μm filtered solution of 20mM HEPES, 150mM NaCl, 2mM MgSO4, 0.1mM ZnCl2,pH 7.5.
Storage Conditions:
Store at -20°C.Store the lyophilized protein at -20°C to -80 °C up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Additional Names:
Alpl, Akp-2, Akp2,Alkaline phosphatase, tissue-nonspecific isozyme, AP-TNAP, TNAP, TNSALP, EC:3.1.3.1, Alkaline phosphatase 2, Alkaline phosphatase liver/bone/kidney isozyme, Phosphoamidase, Phosphocreatine phosphatase, EC:3.9.1.1
Protein Accession Number:
NP_001274101.1
Ncbi Gene Id Number:
11647
Background:
Several distinct genes encode alkaline phosphatases (APs) in mice with different tissue-specific expression patterns. The Alpl gene, also known as Akp2, encodes the liver/bone/kidney isozyme, also known as the tissue-nonspecific AP (TNAP). The Alpl gene is a key regulator of bone mineralization in mice . A variety of mutations in the human ALPL gene leads to different forms of hypophosphatasia, characterized by poorly mineralized cartilage and bones . The native ALPL is a glycosylated homodimer attached to the membrane through a GPI-anchor.

FOR RESEARCH USE ONLY

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Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

85-959

List Size:
0.01 mg, 0.05 mg

List Price:

Price range: $140.00 through $350.00

Datasheet
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