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Recombinant Human/Mouse/Rat Mature BMP-4 Protein was determined by SDS-PAGE under reducing (R) and non-reducing (NR) conditions.
Recombinant Human/Mouse/Rat Mature BMP-4 Protein induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells.The ED<sub>50</sub> for this effect is 5.24-20.96 ng/mL, corresponding to a specific activity of 4.77×10<sup>4</sup>~1.91×10<sup>5</sup> units/mg.
Measured by its binding ability in a functional ELISA. Immobilized Human BMP4 Protein at 5μg/mL (100 μL/well) can bind Recombinant Human RGMA (Catalog:85-007) with a linear range of 10-130 ng/mL.
Recombinant Human/Mouse/Rat Mature BMP-4 Protein was determined by SDS-PAGE under reducing (R) and non-reducing (NR) conditions.

Human/Mouse/Rat Mature BMP-4 Recombinant Protein (85-989)

CATALOG NUMBER: 85-989

Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Specifications
source-species:
CHO cells
Species:
Human,Mouse,Rat
source-species:
CHO cells
Recombinant Protein Sequence:
Ser293-Arg408
Fusion Tag:
No-Tag
Tested Applications:
Bioactivity Test, ELISA, Immunogen, SDS-PAGE, WB
Application Note:
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity Test
Predicted Molecular Weight:
13.13 kDa
Biological Activity:
1.Recombinant Human/Mouse/Rat Mature BMP-4 Protein induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells.The ED50 for this effect is 5.24-20.96 ng/mL, corresponding to a specific activity of 4.77×104~1.91×105 units/mg.2.Measured by its binding ability in a functional ELISA. Immobilized Human BMP4 Protein at 5μg/mL (100 μL/well) can bind Recombinant Human RGMA with a linear range of 10-130 ng/mL.
Purity:
≥ 95 % as determined by SDS-PAGE.
Endotoxin:
< 0.01 EU/μg of the protein by LAL method
physical-state:
Lyophilized
Buffer:
Lyophilized from a 0.22 μm filtered solution of 50mM acetic acid,pH3.6.
Storage Conditions:
Store at -20°C.Store the lyophilized protein at -20°C to -80 °C up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
Additional Names:
BMP4, BMP2B, DVR4,Bone morphogenetic protein 4, BMP-4, Bone morphogenetic protein 2B, BMP-2B
Protein Accession Number:
NP_001193.2
Ncbi Gene Id Number:
652
Background:
Recombinant Human/Mouse/Rat Mature BMP-4 Protein that in humans is encoded by BMP4 gene. BMP4 is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. The superfamily includes large families of growth and differentiation factors. BMP4 is highly conserved evolutionarily. BMP4 is found in early embryonic development in the ventral marginal zone and in the eye, heart blood and otic vesicle.

FOR RESEARCH USE ONLY

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Disclaimer:
Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.

CATALOG NUMBER:

85-989

List Size:
0.01 mg, 0.05 mg

List Price:

Price range: $252.00 through $630.00

Datasheet
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