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Antibody used in IHC on human kidney at 5 ug/ml.
Antibody used in IHC on Human small intestine at 5 ug/ml.
Antibody used in IHC on Human kidney at 5.0 ug/ml.
Antibody used in WB on Human HeLa at 1:300.
Antibody used in WB on Human Hela at: 1:300.
Antibody used in IHC on Human Hela at 1:250.
Antibody used in IHC on human kidney at 5 ug/ml.

ATP6V0A2 Antibody

CATALOG NUMBER: 25-762

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ATP6V0A2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
ATP6V0A2 antibody can be used for detection of ATP6V0A2 by ELISA at 1:312500. ATP6V0A2 antibody can be used for detection of ATP6V0A2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Predicted Molecular Weight:
98 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store ATP6V0A2 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
ATP6V0A2
Additional Names:
ATP6V0A2, ATP6N1D, ATP6a2, J6B7, Stv1, TJ6, TJ6M, TJ6s, Vph1, a2, A2, RTF, WSS, ARCL, STV1, TJ6S, VPH1, ARCL2A, ATP6A2
Protein Accession Number:
NP_036595
Protein Gi Number:
42741679
Ncbi Gene Id Number:
23545
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The multisubunit vacuolar-type proton pump (H (+)-ATPase or V-ATPase) is essential for acidification of diverse cellular components, including endosomes, lysosomes, clathrin-coated vesicles, secretory vesicles, and chromaffin granules, and it is found at high density in the plasma membrane of certain specialized cells. H (+)-ATPases are comprised of a peripheral V (1) domain and an integral membrane V (0) domain; ATP6V0A2 is a component of the V (0) domain.The multisubunit vacuolar-type proton pump (H (+)-ATPase or V-ATPase) is essential for acidification of diverse cellular components, including endosomes, lysosomes, clathrin-coated vesicles, secretory vesicles, and chromaffin granules, and it is found at high density in the plasma membrane of certain specialized cells. H (+)-ATPases are comprised of a peripheral V (1) domain and an integral membrane V (0) domain; ATP6V0A2 is a component of the V (0) domain (Smith et al., 2003 [PubMed 14580332]).
Background Reference 1:
Kornak, U., (2008) Nat. Genet. 40 (1), 32-34.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

25-762

List Size:
100 ul

List Price:

$519.00

Datasheet
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