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Premier

Client-Provided Splenocytes

Time: ~7-12 weeks

2-3 weeks

Phase II: Single-Cell Sorting, Culturing, and Screening
  • Splenocyte isolation from 1 rabbit
  • Sorting and culturing antibody secreting B-cells
  • ELISA screening

4-8 weeks

Phase III: Antibody Cloning and Small-Scale Expression
  • Cloning variable regions from B-cells
  • Transient expression of recombinant antibodies

8-13 weeks

Phase IV: Sequencing, Expression, and Purification
  • Sequencing heavy and light chains
  • Expression and purification of up to 5 antibodies
Deliverables
  • ELISA-positive B-cell supernatants, up to 96
  • ELISA-positive recombinant supernatants, up to 10
  • 100ug purified antibody from up to 5 clones
  • DNA and sequences of up to 5 final clones
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Pro

Client-Provided Antigen

Time: 16-26 weeks

10-15 weeks

Phase I: Immunization
  • 3 Rabbits
  • 1 Pre-immune serum collection
  • 5 Immunizations
  • 2 Serum collections
  • ELISA at each serum collection
  • Splenocyte isolation from 2 rabbits

2-3 weeks

Phase II: Single-cell Sorting, Culturing, and Screening
  • Sorting and culturing antibody secreting B-cells
  • ELISA screening

4-8 weeks

Phase III: Antibody Cloning and Small-Scale Expression
  • Cloning variable regions from B-cells
  • Transient expression of recombinant antibodies

18-23 weeks

Phase IV: Sequencing, Expression, and Purification
  • Sequencing heavy and light chains
  • Expression and purification of up to 5 antibodies
Deliverables
  • ELISA-positive B-cell supernatants, up to 96
  • ELISA-positive recombinant supernatants, up to 10
  • 100ug purified antibody from up to 5 clones
  • DNA and sequences of up to 5 final clones
Get a Quote