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Western blot analysis of anti-CAMK 1G Pab in NCI-H460 cell lysate
Western blot analysis of CAMK1G using rabbit polyclonal CAMK1G Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CAMK1G gene (Lane 2).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot analysis of anti-CAMK 1G Pab in NCI-H460 cell lysate

CAMK1G Antibody

CATALOG NUMBER: 62-763

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This CAMK1G (CaMKI gamma) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 420-450 amino acids from the C-terminal region of human CAMK1G (CaMKI gamma).
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
53 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CAMK1G
Additional Names:
Calcium/calmodulin-dependent protein kinase type 1G, CaM kinase I gamma, CaM kinase IG, CaM-KI gamma, CaMKI gamma, CaMKIG, CaMK-like CREB kinase III, CLICK III, CAMK1G, CLICK3, VWS1
Protein Accession Number:
Q96NX5
Protein Gi Number:
73620970
Ncbi Gene Id Number:
57172
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Ca2+/calmodulin-dependent protein kinase I (CaMKI) constitutes a family of closely related isoforms (alpha, beta and gamma). CLICK-III/CaMKIgamma is a novel membrane-anchored neuronal Ca2+/calmodulin-dependent protein kinase. AMKIgamma is abundant in neurons, particularly in the amygdala and ventromedial hypothalamus. Like the other CaMKI isoforms, full activation of CLICK-III/CaMKIgamma requires both Ca(2+)/CaM and phosphorylation by CaMKK.
Background Reference 1:
Takemoto-Kimura, S., et al., J. Biol. Chem. 278(20):18597-18605 (2003).
Background Reference 2:
Schutte, B.C., et al., Genome Res. 10(1):81-94 (2000).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-763

List Size:
400 ul

List Price:

$495.00

Datasheet
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