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Western blot analysis in CEM cell line lysates (35ug/lane).
CAPN11 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in CEM cell line lysates (35ug/lane).

CAPN11 Antibody

CATALOG NUMBER: 57-319

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This CAPN11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 409-438 amino acids from the Central region of human CAPN11.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
84 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CAPN11
Additional Names:
Calpain-11, 3422-, Calcium-activated neutral proteinase 11, CANP 11, CAPN11
Protein Accession Number:
Q9UMQ6
Protein Gi Number:
218511961
Ncbi Gene Id Number:
11131
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Calpains constitute a family of intracellularcalcium-dependent cysteine proteases. There are eight members inthis superfamily. They consist of a variable 80 kDa subunit and aninvariant 30 kDa subunit. This calpain protein appears to haveprotease activity and calcium-binding ability. A similar mouseprotein may play a functional role in spermatogenesis and in theregulation of calcium-dependent signal transduction events duringmeiosis.
Background Reference 1:
Ben-Aharon, I., et al. Mol. Reprod. Dev. 73(6):767-773(2006)
Background Reference 2:
Dear, T.N., et al. Genomics 59(2):243-247(1999)
Background Reference 3:
Rojas, F.J., et al. Mol. Hum. Reprod. 5(6):520-526(1999)
Background Reference 4:
Saido, T.C., et al. FASEB J. 8(11):814-822(1994)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-319

List Size:
400 ul

List Price:

$495.00

Datasheet
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