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![Western blot analysis of various lysates using [KO Validated] PTEN Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 1s.](https://www.prosciantibodies.com/wp-content/uploads/abclonal/ARC2643_WB_01_81-373.jpg)
![Western blot analysis of lysates from wild type (WT) and Rbm7 knockout (KO) mouse testis using [KO Validated] Rbm7 Rabbit mAb at 1:5000 dilution incubated at room temperature for 1.5 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 60s.|WB samples for antibody validation are kindly provided by Dr. Shaorong Gao.](https://www.prosciantibodies.com/wp-content/uploads/abclonal/ARC73985-01_WB_02_83-542.jpg)
![Western blot analysis of lysates from wild type (WT) and RhoGDI knockout (KO) 293T cells, using [KO Validated] RhoGDI Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 30s.](https://www.prosciantibodies.com/wp-content/uploads/abclonal/ARC0629_KO-WB_01_81-181.jpg)
![Western blot analysis of lysates from Hep G2 cells using [KO Validated] SIRT3 Rabbit mAb at 1:1000 dilution incubated overnight at 4℃.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 90s.](https://www.prosciantibodies.com/wp-content/uploads/abclonal/ARC51535-02_WB_01_82-244.jpg)
![Western blot analysis of lysates from MCF7 cells, using [KO Validated] SLC25A4/ANT1 Rabbit mAb at1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 10s.](https://www.prosciantibodies.com/wp-content/uploads/abclonal/ARC51442-01_WB_01_81-729.jpg)
![Western blot analysis of various lysates using [KO Validated] SLC25A4/ANT1 Rabbit mAb at 1:1000 dilution.| Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.| Lysates / proteins: 25 μg per lane.| Blocking buffer: 3 % nonfat dry milk in TBST.| Detection: ECL Basic Kit.| Exposure time: 10s.](https://www.prosciantibodies.com/wp-content/uploads/abclonal/ARC51433-01_WB_01_83-109.jpg)
![Western blot analysis of various lysates using [KO Validated] Smad1 Rabbit mAb at1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 90s.](https://www.prosciantibodies.com/wp-content/uploads/abclonal/ARC52106-01_WB_01_82-194.jpg)
![Western blot analysis of lysates from wild type (WT) and Smad1 knockout (KO) HeLa cells, using [KO Validated] Smad1 Rabbit mAb at 1:1000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 30s.](https://www.prosciantibodies.com/wp-content/uploads/abclonal/ARC0365_KO-WB_01_82-640.jpg)
