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Western blot analysis using CD19 monoclonal antibody against truncated CD19 recombinant protein.
Immunohistochemical analysis of paraffin - embedded human normal lymph node showing cytoplasmic localization using CD19 antibody with DAB staining.
Western blot analysis using CD19 monoclonal antibody against truncated CD19 recombinant protein.

CD19 Antibody [2E2B6B10]

CATALOG NUMBER: 32-129

Clonality:
Monoclonal
Tested Applications:
IHC, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Ni-NTA purified recombinant human CD19 expressed in E. Coli strain M15.
Conjugate:
Unconjugated
Tested Applications:
IHC, WB
Application Note:
Western Blot:Dilution 1:2000 - 5,000
IHC(P):Dilution 1:100 - 1:400
IHC(F):Dilution 1:200 - 1:400
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Purification:
Antibody is purified by protein A affinity chromatography.
Clonality:
Monoclonal
Isotype:
IgG2a
Buffer:
Purified antibody in PBS containing 0.03% sodium azide.
Concentration:
batch dependent
Storage Conditions:
CD19 monoclonal antibody can be stored at -20°C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CD19
Additional Names:
B-lymphocyte surface antigen B4B4, CVID3
Protein Accession Number:
P15391
Protein Gi Number:
160332376
Ncbi Gene Id Number:
930
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The CD19 antigen (95 kDa) is expressed from the earliest stage of B progenitor development, on all peripheral B cells including germinal centre B cells, and all B cell lines and B cell leukaemia tested. T cell and monocytic cell lines are negative and the antigen is lost on B cell maturation to plasma cells. The antigen is a type I integral membrane glycoprotein whose in vitro inhibition will influence B cell activation and proliferation.
Background Reference 1:
Rie, M.A. de, J. of Immunol. Methods, 1987. 102: 187.
Background Reference 2:
Rie, M.A. de, Leukaemia Research, 1988. 12: 135.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

32-129

List Size:
0.1 mg

List Price:

$302.00

Datasheet
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