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42-633 (0.01ug/ml) staining of Mouse Testis lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
42-633 (3.8ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
42-633 (0.01ug/ml) staining of Mouse Testis lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

CIRBP Antibody

CATALOG NUMBER: 42-633

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Homology:
Expected Species Reactivity based on sequence homology: Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-RDSYDSYATHNE
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 18kDa band observed in Mouse Testis lysates (calculated MW of 18.6kDa according to Human NP_001271.1 and Mouse NP_031731.1). Recommended concentration: 0.01-0.03ug/ml. Primary incubation was 1 hour.Immunohistochemistry:In paraffin embedded Human Testis shows nuclear staining preferentially in type A spermatogonia. Recommended concentration: 3-5ug/ml.
Specificity:
This antibody is expected to recognize reported isoform 1 (NP_001271.1) only.
Positive Control 1:
Cat. No. 1416 - Mouse Testis Tissue Lysate
Positive Control 2:
Cat. No. 1476 - Rat Testis Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
CIRBP
Additional Names:
CIRBP, cold inducible RNA binding protein, CIRP, Cold-inducible RNA-binding protein, cold inducible RNA-binding protein, glycine-rich RNA binding protein
Protein Accession Number:
NP_001271.1
Protein Gi Number:
4502847
Ncbi Gene Id Number:
1153

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-633

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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