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Western blot analysis in A549 cell line lysates (35ug/lane).
CMGA Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Confocal immunofluorescent analysis of CMGA Antibody with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in A549 cell line lysates (35ug/lane).

CMGA Antibody

CATALOG NUMBER: 56-038

Clonality:
Polyclonal
Tested Applications:
Flow, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This CMGA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 376-404 amino acids from the C-terminal region of human CMGA.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
51 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CHGA
Additional Names:
Chromogranin-A, CgA, Pituitary secretory protein I, SP-I, Vasostatin-1, Vasostatin I, Vasostatin-2, Vasostatin II, EA-92, ES-43, Pancreastatin, SS-18, WA-8, WE-14, LF-19, AL-11, GV-19, GR-44, ER-37, CHGA
Protein Accession Number:
P10645
Protein Gi Number:
215274270
Ncbi Gene Id Number:
1113
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a member of thechromogranin/secretogranin family of neuroendocrine secretoryproteins. It is found in secretory vesicles of neurons andendocrine cells. This gene product is a precursor to threebiologically active peptides; vasostatin, pancreastatin, andparastatin. These peptides act as autocrine or paracrine negativemodulators of the neuroendocrine system. Other peptides, includingchromostatin, beta-granin, WE-14 and GE-25, are also derived fromthe full-length protein. However, biological activities for thesemolecules have not been shown.
Background Reference 1:
Ezzi, S.A., et al. J. Neurochem. 115(5):1102-1111(2010)
Background Reference 2:
Ma, Z., et al. J. Urol. 184(3):1182-1188(2010)
Background Reference 3:
Ramella, R., et al. J. Cell. Biochem. 110(1):70-79(2010)
Background Reference 4:
Dag, E., et al. Peptides 31(5):932-937(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-038

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

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