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Western Blot at 1:2000 dilution Lane 1: A375 whole cell lysate Lane 2: human brain lysate Lane 3: human liver lysate Lysates/proteins at 20 ug per lane.
Formalin-fixed and paraffin-embedded human brain tissue reacted with DLL3 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Confocal immunofluorescent analysis of DLL3 Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Western Blot at 1:2000 dilution Lane 1: A375 whole cell lysate Lane 2: human brain lysate Lane 3: human liver lysate Lysates/proteins at 20 ug per lane.

DLL3 Antibody

CATALOG NUMBER: 63-900

Clonality:
Polyclonal
Tested Applications:
IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This DLL3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 519-548 amino acids from the C-terminal region of human DLL3.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:2000
For IHC-P starting dilution is: 1:50~100
For IF starting dilution is: 1:10~50
Predicted Molecular Weight:
65 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
DLL3
Additional Names:
Delta-like protein 3, Drosophila Delta homolog 3, Delta3, DLL3
Protein Accession Number:
Q9NYJ7
Protein Gi Number:
12229810
Ncbi Gene Id Number:
10683
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
DLL3 encodes a member of the delta protein ligand family. This family functions as Notch ligands that are characterized by a DSL domain, EGF repeats, and a transmembrane domain.
Background Reference 1:
Yerges,L.M. J. Bone Miner. Res. 24 (12), 2039-2049 (2009)
Background Reference 2:
Heuss,S.F. Proc. Natl. Acad. Sci. U.S.A. 105 (32), 11212-11217 (2008)
Background Reference 3:
Maisenbacher,M.K. Hum. Genet. 116 (5), 416-419 (2005)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-900

List Size:
200 ul

List Price:

$495.00

Datasheet
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