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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells labeling Pdx1 with antibody at 1/25 dilution, followed by Dylight 488-conjugated goat anti-rabbit IgG (NK179883) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HepG2 cell line. The nuclear counter stain is DAPI (blue).
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells labeling Pdx1 with antibody at 1/25 dilution, followed by Dylight 488-conjugated goat anti-rabbit IgG (NK179883) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HepG2 cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin (PD18466410) at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).
Antibody staining DVL1 in human skeletal muscle sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Western Blot at 1:2000 dilution Lane 1: K562 whole cell lysate Lane 2: MDA-MB-453 whole cell lysate Lane 3: PC-3 whole cell lysate Lane 4: human kidney lysate Lane 5: NIH/3T3 whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:1000 dilution + PC-3 whole cell lysates Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution Lane 1: K562 whole cell lysates Lane 2: MDA-MB-453 whole cell lysates Lane 3: PC-3 whole cell lysates Lane 4: human kidney lysates Lysates/proteins at 20 ug per lane.
Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells labeling Pdx1 with antibody at 1/25 dilution, followed by Dylight 488-conjugated goat anti-rabbit IgG (NK179883) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HepG2 cell line. The nuclear counter stain is DAPI (blue).

DVL1 Antibody

CATALOG NUMBER: 56-349

Clonality:
Polyclonal
Tested Applications:
IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Rat
Immunogen:
This DVL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 442-470 amino acids from the Central region of human DVL1.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC-P, WB
Application Note:
For IF starting dilution is: 1:25
For IHC-P starting dilution is: 1:25
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
75 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
DVL1
Additional Names:
Segment polarity protein dishevelled homolog DVL-1, Dishevelled-1, DSH homolog 1, DVL1
Protein Accession Number:
O14640
Ncbi Gene Id Number:
1855
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
DVL1, the human homolog of the Drosophila dishevelled gene(dsh) encodes a cytoplasmic phosphoprotein that regulates cellproliferation, acting as a transducer molecule for developmentalprocesses, including segmentation and neuroblast specification.DVL1 is a candidate gene for neuroblastomatous transformation. TheSchwartz-Jampel syndrome and Charcot-Marie-Tooth disease type 2Ahave been mapped to the same region as DVL1. The phenotypes ofthese diseases may be consistent with defects which might beexpected from aberrant expression of a DVL gene during development.
Background Reference 1:
Metcalfe, C., et al. J. Cell. Sci. 123 (PT 9), 1588-1599 (2010) :
Background Reference 2:
Hu, T., et al. J. Biol. Chem. 285(18):13561-13568(2010)
Background Reference 3:
Varelas, X., et al. Dev. Cell 18(4):579-591(2010)
Background Reference 4:
Jugessur, A., et al. PLoS ONE(7), E11493 (2010) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-349

List Size:
400 ul

List Price:

$495.00

Datasheet
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