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To a sandwich ELISA (assuming 100ul/well), a concentration of 2.0-4.0 ug/ml of this antibody will detect at least 80.0 pg/well of recombinant human Eotaxin-2 when used with ProSci's biotinylated antigen affinity purified anti-human Eotaxin-2 (XP-5126Bt) as the detection antibody at a concentration of approximately 1.0-2.0 ug/ml.
To detect hEotaxin-2 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEotaxin-2 is 0.5-1.0 ng/lane, under non-reducing conditions.
To detect hEotaxin-2 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEotaxin-2 is 0.5-1.0 ng/lane, under non-reducing conditions.
This antibody stained formalin-fixed, paraffin-embedded sections of human tonsil. The recommended concentration is 10.0 ug/ml with an overnight incubation at 4C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 8.0 EDTA buffer is recommended. Optimal concentrations and conditions may vary. Protocol and staining provided by Dr. Lauren Binge, Laboratory of Prof. Charles Mackay, Monash Univeristy, Australia.
This antibody stained formalin-fixed, paraffin-embedded sections of human tonsil. The recommended concentration is 10.0 ug/ml with an overnight incubation at 4C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 8.0 EDTA buffer is recommended. Optimal concentrations and conditions may vary. Protocol and staining provided by Dr. Lauren Binge, Laboratory of Prof. Charles Mackay, Monash Univeristy, Australia.
This antibody stained formalin-fixed, paraffin-embedded sections of human tonsil. The recommended concentration is 10.0 ug/ml with an overnight incubation at 4C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 8.0 EDTA buffer is recommended. Optimal concentrations and conditions may vary. Protocol and staining provided by Dr. Lauren Binge, Laboratory of Prof. Charles Mackay, Monash Univeristy, Australia.
To a sandwich ELISA (assuming 100ul/well), a concentration of 2.0-4.0 ug/ml of this antibody will detect at least 80.0 pg/well of recombinant human Eotaxin-2 when used with ProSci's biotinylated antigen affinity purified anti-human Eotaxin-2 (XP-5126Bt) as the detection antibody at a concentration of approximately 1.0-2.0 ug/ml.

Eotaxin-2 Antibody [1.1_6C7-2C5]

CATALOG NUMBER: XP-5715-M

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
The monoclonal antibody was produced in BALB/c X ICR F1 mice using recombinant human Eotaxin-2 as the immunizing antigen.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
ELISA:
In a sandwich ELISA (assuming 100 μL/well), a concentration of 2.0-4.0 μg/mL of this antibody will detect at least 80.0 pg/well of recombinant human Eotaxin-2 when used with our biotinylated antigen affinity purified anti-human Eotaxin-2 (XP-5126Bt) as the detection antibody at a concentration of approximately 1.0-2.0 μg/mL.
Western blot:
To detect hEotaxin-2 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEotaxin-2 is 0.5-1.0 ng/lane, under non-reducing conditions.
Purification:
This IgG1K antibody was purified from ascites fluid followed by Protein A affinity chromatography.
Clonality:
Monoclonal
Physical State:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
Eotaxin-2 lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4°C when reconstituted.
Ncbi Official Symbol:
CCL24
Additional Names:
MPIF2, SCYA24, C-C motif chemokine 24, CK-beta-6, MPIF-2, Ckb-6
Protein Accession Number:
O00175
Protein Gi Number:
6920081
Ncbi Gene Id Number:
6369
User Note:
Centrifuge vial prior to opening.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

XP-5715-M

List Size:
0.5 mg

List Price:

$376.00

Datasheet
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