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Western blot analysis of F1A alpha in mouse (A) and rat (B) liver tissue lysates with F1A alpha antibody at 1 μg/mL.
Immunohistochemistry of F1Aα in mouse liver tissue with F1Aα antibody at 5 μg/mL.
Immunofluorescence of F1A alpha in Mouse Liver cells with F1A alpha antibody at 20 μg/mL.
Immunofluorescence of F1A alpha in mouse liver tissue with F1A alpha antibody at 20 μg/ml.<br><br>Green: F1A alpha Antibody (2279) <br> Blue: DAPI staining
Western blot analysis of F1A alpha in mouse (A) and rat (B) liver tissue lysates with F1A alpha antibody at 1 μg/mL.
Green Product
Trial Size

F1A alpha Antibody

CATALOG NUMBER: 2279

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Chicken: (79%)
Immunogen:
F1A antibody was raised against a 14 amino acid peptide near the carboxy terminus of human F1A.
The immunogen is located within the last 50 amino acids of F1A alpha.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
F1Ab antibody can be used for detection of F1Ab by Western blot at 1 μg/mL. An approximately 70 kDa band can be detected. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse and rat samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1404 - Mouse Liver Tissue Lysate
Positive Control 2:
Cat. No. 1464 - Rat Liver Tissue Lysate
Predicted Molecular Weight:
70 kDa
Purification:
F1A alpha Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
F1A alpha Antibody is supplied in PBS containing ≤0.09% sodium azide.
Concentration:
1 mg/ml
Storage Conditions:
F1A alpha antibody can be stored at 4°C, stable for up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
FEM1B
Additional Names:
F1A alpha Antibody: F1AA, F1A-ALPHA, FEM1-beta, F1AA, KIAA0396, Protein fem-1 homolog B, FEM1b
Protein Accession Number:
AAF05314
Protein Gi Number:
6175869
Ncbi Gene Id Number:
10116
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
F1A alpha Antibody: Fas and tumor necrosis factor receptor 1 (TNFR1) are two prototype members in the death receptor family. A novel protein that associates with the intracellular domains of Fas and TNFR1 was recently identified and designated F1Aalpha and FEM1β. F1Aalpha/FEM1β is the homologue of C. elegans sex determining protein FEM-1. FEM-1/F1Aalpha is cleaved by CED-3 and caspase. FEM-1/F1Aalpha associates with CED-4 and its mammalian homologue Apaf-1. Overexpression of F1Aalpha induces apoptosis. F1Aalpha is therefore a novel member of the death receptor associated protein that mediates apoptosis. F1Aalpha is expressed in a variety of human and mouse tissues.
Background Reference 1:
Chan SL, Tan KO, Zhang L, Yee KS , Ronca F, Chan MY, Yu VC. F1Aα, a death receptor-binding protein homologous to the Caenorhabditis elegans sex-determining protein, FEM-1, is a caspase substrate that mediates apoptosis. J Biol Chem. 1999;274(45):32461-8.
Background Reference 2:
Ventura-Holman T, Maher JF. Sequence, organization, and expression of the human FEM1B gene. Biochem Biophys Res Commun 2000;267(1):317-20
Background Reference 3:
Chan SL, Yee KS , Tan KM, Yu VC. The Caenorhabditis elegans sex determination protein FEM-1 is a CED-3 substrate that associates with CED-4 and mediates apoptosis in mammalian cells. J Biol Chem. 2000;275(24):17925-8. (WD0102)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

2279

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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