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Western blot analysis of FRAT1 using rabbit polyclonal FRAT1 Antibody.293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the FRAT1 gene (Lane 2).
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with FRAT1 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of FRAT1 using rabbit polyclonal FRAT1 Antibody.293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the FRAT1 gene (Lane 2).

FRAT1 Antibody

CATALOG NUMBER: 57-684

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This FRAT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 99-128 amino acids from the Central region of human FRAT1.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
29 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
FRAT1
Additional Names:
Proto-oncogene FRAT1, Frequently rearranged in advanced T-cell lymphomas 1, FRAT-1, FRAT1
Protein Accession Number:
Q92837
Protein Gi Number:
51338813
Ncbi Gene Id Number:
10023
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
FRAT1 belongs to the GSK-3-binding protein family. It may function in tumor progression and in lymphomagenesis. It positively regulates the Wnt signaling pathway by stabilizing beta-catenin through the association with GSK-3. It may play a role in tumor progression and collaborate with PIM1 and MYC in lymphomagenesis.
Background Reference 1:
Hagen,T., J. Biol. Chem. 281 (46), 35021-35029 (2006)
Background Reference 2:
Wang,Y., Br. J. Cancer 94 (5), 686-691 (2006)
Background Reference 3:
Hino,S., J. Biol. Chem. 278 (16), 14066-14073 (2003)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-684

List Size:
400 ul

List Price:

$495.00

Datasheet
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