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Western blot analysis of lysed extracts from 3T3 cells using GATA1 (Ab-142).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GATA1 (Ab-142).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using GATA1 (Ab-142).
Western blot analysis of lysed extracts from 3T3 cells using GATA1 (Ab-142).

GATA1 (Ab 142) Antibody

CATALOG NUMBER: 79-284

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
GATA1 (Ab-142) antibody was raised against a peptide sequence around aa.140~144 (R-L-S-P-D) derived from Human GATA1.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Specificity:
This antibody detects endogenous level of total GATA1 protein.
Predicted Molecular Weight:
43 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
GATA1
Additional Names:
GF1, GF-1, NFE1, XLTT, ERYF1, NF-E1, XLANP, XLTDA, GATA-1, PLC1, GAT1, GATA1
Protein Accession Number:
NP_002040.1
Protein Gi Number:
4503925
Ncbi Gene Id Number:
2623
Background:
GATA1 encodes a protein which belongs to the GATA family of transcription factors. The protein plays an important role in erythroid development by regulating the switch of fetal hemoglobin to adult hemoglobin. Mutations in this gene have been associated with X-linked dyserythropoietic anemia and thrombocytopenia.
Background Reference 1:
Zon LI, et al. (1990) Proc Natl Acad. Sci USA. 87: 668-672. Trainor C D, et al. (1990) Nature. 343: 92-96. Nichols K E, et al. (2000) Nat Genet. 24: 266-270. Freson K, et al. (2001) Blood. 98: 85-92. Mehaffey M G, et al. (2001) Blood. 98: 2681-2688.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-284

List Size:
0.1 mg

List Price:

$370.00

Datasheet
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