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45-065 (1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-065 (3.8ug/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-065 (20ug/ml) staining of PFA-perfused cryosection of Porcin Kidney. Microwave antigen retrieval with citrate buffer pH 3, CY3-staining. Data obtained from Dr. Hrvoje Brzica, University of Zagreb, Croatia
45-065 (3.75ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
45-065 (1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Glutathione Peroxidase 1 Antibody

CATALOG NUMBER: 45-065

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Pig
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Pig
Immunogen:
The immunogen for this antibody is: C-REALPAPSDDATA
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 22kDa band observed in Human Liver lysates (calculated MW of 22.1kDa according to NP_000572.2). Recommended concentration: 1-3ug/ml.Immunohistochemistry:In paraffin embedded Human Cerebral Cortex shows staining of microglia. Recommended concentration: 3-5ug/ml. Paraffin embedded Human Liver. Recommended concentration: 3.75ug/ml.Frozen sections of Porcin Kidney shows staining of cytoplasm in PCT epithelial cells. Recommended concentration: 20ug/ml.
Specificity:
This antibody is expected to recognise isoform 1 ( NP_000572.2) only.
Positive Control 1:
Cat. No. 1304 - Human Liver Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
GPX1
Additional Names:
glutathione peroxidase 1 , HGNC:4553, GPX1, GSHPX1, MGC14399, MGC88245
Protein Accession Number:
NP_000572.2
Protein Gi Number:
41406084
Ncbi Gene Id Number:
2876
Background Reference 1:
Kato S, Kato M, Abe Y, Matsumura T, Nishino T, Aoki M, Itoyama Y, Asayama K, Awaya A, Hirano A, Ohama E. Redox system expression in the motor neurons in amyotrophic lateral sclerosis (ALS): immunohistochemical studies on sporadic ALS, superoxide dismutase 1 (SOD1)-mutated familial ALS, and SOD1-mutated ALS animal models. Acta Neuropathol (Berl). 2005 Jun 28;

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-065

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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